ArticleNature plants2020
The active site of magnesium chelatase.
Article in Nature plants, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- The structural chemistry and biosynthesis of chlorophylls.RSC chemical biology · 2026Review
- Comparative transcriptomics and targeted metabolomics reveal mechanisms of leaf greenness retention during tobacco (Nicotiana tabacum L.) air-curing.BMC plant biology · 2026Article
- Commensal gut bacteria employ de-chelatase HmuS to harvest iron from heme.The EMBO journal · 2025Article
- Characterization of a tomato chlh mis-sense mutant reveals a new function of ChlH in fruit ripening.Plant biotechnology journal · 2025Article
- A missense mutation in the barley Xan-h gene encoding the Mg-chelatase subunit I leads to a viable pale green line with reduced daily transpiration rate.Plant cell reports · 2024Article
- Analysis of Hyperosmotic Tolerance Mechanisms inGenes · 2024Article
- QTL mapping and candidate gene analysis reveal two major loci regulating green leaf color in non-heading Chinese cabbage.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Mutagenesis selection and large-scale cultivation of non-greenFrontiers in nutrition · 2024Article
- Article
- Engineering Chlorophyll, Bacteriochlorophyll, and Carotenoid Biosynthetic Pathways inACS synthetic biology · 2023Article
- Endophytes in Agriculture: Potential to Improve Yields and Tolerances of Agricultural Crops.Microorganisms · 2023Review
- Absolute quantification of cellular levels of photosynthesis-related proteins in Synechocystis sp. PCC 6803.Photosynthesis research · 2023Article
- Crystal structure and reaction mechanism of a bacterial Mg-dechelatase homolog from the Chloroflexi Anaerolineae.Protein science : a publication of the Protein Society · 2022Article
- The terminal enzymes of (bacterio)chlorophyll biosynthesis.Royal Society open science · 2022Review
- Fine Mapping and Characterization of a Major Gene Responsible for Chlorophyll Biosynthesis inBiomolecules · 2022Article
- Impact of Porphyrin Binding to GENOMES UNCOUPLED 4 on Tetrapyrrole BiosynthesisFrontiers in plant science · 2022Article
- Chloroplast SRP43 autonomously protects chlorophyll biosynthesis proteins against heat shock.Nature plants · 2021Article
- Insights into the structure and function of the rate-limiting enzyme of chlorophyll degradation through analysis of a bacterial Mg-dechelatase homolog.Computational and structural biotechnology journal · 2021Article
- The Role of Membranes and Lipid-Protein Interactions in the Mg-Branch of Tetrapyrrole Biosynthesis.Frontiers in plant science · 2021Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The insertion of magnesium into protoporphyrin initiates the biosynthesis of chlorophyll, the pigment that underpins photosynthesis. This reaction, catalysed by the magnesium chelatase complex, couples ATP hydrolysis by a ChlID motor complex to chelation within the ChlH subunit. We probed the structure and catalytic function of ChlH using a combination of X-ray crystallography, computational modelling, mutagenesis and enzymology. Two linked domains of ChlH in an initially open conformation of ChlH bind protoporphyrin IX, and the rearrangement of several loops envelops this substrate, forming an active site cavity. This induced fit brings an essential glutamate (E660), proposed to be the key catalytic residue for magnesium insertion, into proximity with the porphyrin. A buried solvent channel adjacent to E660 connects the exterior bulk solvent to the active site, forming a possible conduit for the delivery of magnesium or abstraction of protons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.