ArticleBMC genomics2020
Virulence characterization and comparative genomics of Listeria monocytogenes sequence type 155 strains.
Article in BMC genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 56 citations in OpenAlex.
- Hybrid Genome Sequencing and Comparative Analysis of Three NovelFoods (Basel, Switzerland) · 2025Article
- Environmental monitoring and equipment sanitation to mitigateApplied and environmental microbiology · 2025Article
- High density genomic surveillance and risk profiling of clinical Listeria monocytogenes subtypes in Germany.Genome medicine · 2024Article
- Whole Genome Sequence Analysis ofMicroorganisms · 2024Article
- Unveiling the Mutations and Conservation of InlA inMicroorganisms · 2024Article
- Characterization ofAntibiotics (Basel, Switzerland) · 2024Article
- Detection of Pathogenic Serogroups and Virulence Genes inInternational journal of microbiology · 2024Article
- Antimicrobial resistance and genome characteristics of Salmonella enteritidis from Huzhou, China.PloS one · 2024Article
- Genetic diversity ofAIMS microbiology · 2024Article
- Surveillance and genetic characterization ofFrontiers in microbiology · 2024Article
- A Comprehensive Virulence and Resistance Characteristics ofInternational journal of molecular sciences · 2023Article
- Sugar Modification of Wall Teichoic Acids Determines Serotype-Dependent Strong Biofilm Production in Listeria monocytogenes.Microbiology spectrum · 2022Article
- Article
- Phylogenetic and Phenotypic Analyses of a Collection of Food and Clinical Listeria monocytogenes Isolates Reveal Loss of Function of Sigma B from Several Clonal Complexes.Applied and environmental microbiology · 2022Article
- Genetic Diversity and Relationships ofMicroorganisms · 2022Article
- Genomic characteristics and comparative genomics of Salmonella enterica subsp. enterica serovar Schwarzengrund strain S16 isolated from chicken feces.Gut pathogens · 2022Article
- The population structure and genetic diversity ofFrontiers in microbiology · 2022Article
- SARS-CoV-2 surveillance in Italy through phylogenomic inferences based on Hamming distances derived from pan-SNPs, -MNPs and -InDels.BMC genomics · 2021Article
- Application of Whole Genome Sequencing to Understand Diversity and Presence of Genes Associated with Sanitizer Tolerance inFoods (Basel, Switzerland) · 2021Article
- Whole-Genome Sequencing-Based Characterization of aMicroorganisms · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
backgroundListeria (L.) monocytogenes strains show a high diversity regarding stress tolerance and virulence potential. Genome studies have mainly focused on specific sequence types (STs) predominantly associated with either food or human listeriosis. This study focused on the prevalent ST155, showing equal distribution among clinical and food isolates. We evaluated the virulence potential of 20 ST155 strains and performed comparative genomic analysis of 130 ST155 strains isolated from food, food processing environments and human listeriosis cases in different countries and years.
resultsThe in vitro virulence assays using human intestinal epithelial Caco2 and hepatocytic HEPG2 cells showed an impaired virulence phenotype for six of the 20 selected ST155 strains. Genome analysis revealed no distinct clustering of strains from the same source category (food, food processing environment, and clinical isolates). All strains harbored an intact inlA and inlB locus, except four strains, which had an internal deletion in the inlA gene. All strains harbored LIPI-1, but prfA was present in a longer variant in six strains, all showing impaired virulence. The longer PrfA variant resulted in lower expression of inlA, inlB, and prfA, and no expression of hly and actA. Regarding stress-related gene content, SSI-1 was present, whereas qacH was absent in all strains. 34.6% of the strains harbored a plasmid. All but one ST155 plasmids showed high conservation and harbored cadA2, bcrABC, and a triphenylmethane reductase.
conclusionsThis study contributes to an enhanced understanding of L. monocytogenes ST155 strains, being equally distributed among isolates from humans, food, and food processing environments. The conservation of the present genetic traits and the absence of unique inherent genetic features makes these types of STs especially interesting since they are apparently equally adapted to the conditions in food processing environments, as well as in food as to the human host environment. However, a ST155-specific mutation resulting in a longer PrfA variant impaired the virulence potential of several ST155 strains.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.