Evidence map›Paper›PMID 33255574›Full record

ArticleMicroorganisms2020

The Duration of Increased Grain Feeding Affects the Microbiota throughout the Digestive Tract of Yearling Holstein Steers.

J C Plaizier, P Azevedo, B L Schurmann, P Górka, G B Penner, E Khafipour

Abstract read
In one paragraph

Article in Microorganisms, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
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  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

J C PlaizierDepartment of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
P AzevedoDepartment of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
B L SchurmannDepartment of Animal and Poultry Science, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada.
P GórkaDepartment of Animal Nutrition and Biotechnology, and Fisheries, University of Agriculture in Krakow, 31-059 Krakow, Poland.ORCID 0000-0002-4278-0493
G B PennerDepartment of Animal and Poultry Science, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada.
E KhafipourDepartment of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.ORCID 0000-0003-4673-7633

Funding

Natural Sciences and Engineering Research Council of Canada RGPIN-2019-05835
6 · The paper itself

Abstract

Effects of the duration of moderate grain feeding on the taxonomic composition of gastrointestinal microbiota were determined in 15 Holstein yearling steers. Treatments included feeding a diet of 92% dry matter (DM) hay (D0), and feeding a 41.5% barley grain diet for 7 (D7) or 21 d (D21) before slaughter. At slaughter, digesta samples were collected from six regions, i.e., the rumen, jejunum, ileum, cecum, colon, and rectum. Extracted DNA from these samples was analyzed using MiSeq Illumina sequencing of the V4 region of the 16S rRNA gene. Three distinct PCoA clusters existed, i.e., the rumen, the jejunum/ileum, and the cecum/colon/rectum. Feeding the grain diet for 7 d reduced microbial diversity in all regions, except the ileum. Extending the duration of grain feeding from 7 to 21 d did not affect this diversity further. Across regions, treatment changed the relative abundances of 89 genera. Most of the changes between D0 and D7 and between D7 and D21 were opposite, demonstrating the resilience of gastrointestinal microbiota to a moderate increase in grain feeding. Results show that the duration of a moderate increase in grain feeding affects how gastrointestinal microbiota respond to this increase.

Indexed as

digestive tractgrainIllumina sequencingmicrobiota

Identifiers

PMID33255574
PMCPMC7761415

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.