ArticleMaterials (Basel, Switzerland)2020
Behaviour of Human Oral Epithelial Cells Grown on Invisalign
Article in Materials (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Clinical outcomes of arch expansion with Invisalign: a systematic review.BMC oral health · 2023Pooled it
- A Dual Cell-Line Molecular Platform to Assess Sub-Threshold Biocompatibility: Application to Processed Orthodontic Clears Aligners.Orthodontics & craniofacial research · 2026Article
- Impact of Zirconia and Titanium Implant Surfaces of Different Roughness on Oral Epithelial Cells.Dentistry journal · 2026Article
- Cytotoxicity evaluation of different clear aligner systems on human primary gingival fibroblasts: an in vitro study.Biomaterial investigations in dentistry · 2026Article
- Cytotoxicity and Endocrine Disruption in Materials Used for Removable Orthodontic Retainers: A Comprehensive Review.Dentistry journal · 2025Review
- A comparative study of the epithelial regeneration capacities of two biomaterials in vitro.BMC oral health · 2025Article
- Risk factors for open gingival embrasures after clear aligners treatment: a retrospective study.BMC oral health · 2025Article
- The cytotoxicity assessment of different clear aligner materials.Journal of dental sciences · 2024Article
- Effects of the Saliva of Patients Undergoing Orthodontic Treatment with Invisalign and Brackets on Human Gingival Fibroblasts and Oral Epithelial Cells.Journal of clinical medicine · 2023Article
- The incidence and severity of open gingival embrasures in adults treated with clear aligners and fixed appliances: a retrospective cohort study.Head & face medicine · 2023Article
- New Materials and Techniques for Orthodontics.Materials (Basel, Switzerland) · 2023Article
- Effect of Titanium and Zirconia Nanoparticles on Human Gingival Mesenchymal Stromal Cells.International journal of molecular sciences · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Invisalign aligners have been widely used to correct malocclusions, but their effect on oral cells is poorly known. Previous research evaluated the impact of aligners' eluates on various cells, but the cell behavior in direct contact with aligners is not yet studied. In the present study, we seeded oral epithelial cells (cell line Ca9-22) directly on Invisalign SmartTrack material. This material is composed of polyurethane and co-polyester and exhibit better mechanical characteristics compared to the predecessor. Cell morphology and behavior were investigated by scanning electron microscopy and an optical cell moves analyzer. The effect of aligners on cell proliferation/viability was assessed by cell-counting kit (CCK)-8 and 3,4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide (MTT) assay and live/dead staining. The expression of inflammatory markers and proteins involved in epithelial barrier function was measured by qPCR. Cells formed cluster-like structures on aligners. The proliferation/viability of cells growing on aligners was significantly lower (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.