Evidence map›Paper›PMID 33253831›Full record

ReviewThe international journal of biochemistry & cell biology2021

Recent advances in nonlinear microscopy: Deep insights and polarized revelations.

A A Gopal, A Kazarine, J M Dubach, P W Wiseman

Open access · greenAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in The international journal of biochemistry & cell biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.4field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. APL bioengineering · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

A A GopalCenter for Systems Biology and Institute for Innovation in Imaging, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA; Department of Chemistry, McGill University, Montreal, Quebec, Canada.
A KazarineDepartment of Chemistry, McGill University, Montreal, Quebec, Canada.
J M DubachCenter for Systems Biology and Institute for Innovation in Imaging, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
P W WisemanDepartment of Chemistry, McGill University, Montreal, Quebec, Canada; Department of Physics, McGill University, Montreal, Quebec, Canada. Electronic address: paul.wiseman@mcgill.ca.
Center for Systems Biology · USMcGill University · CA

Funding

Optimizing PARP inhibitor therapy through novel approaches to resolve the molecular mechanisms of responseR01CA241179 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI DUBACH, JOHN · 2019 to 2023
$1.9M
The role of PARP inhibitor target engagement in breast cancerR00CA198857 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI DUBACH, JOHN · 2017 to 2019
$738k
NCI NIH HHS R00 CA198857NCI NIH HHS R01 CA241179
6 · The paper itself

Abstract

Nonlinear microscopy is a technique that utilizes nonlinear interactions between light and matter to image fluorescence and scattering phenomena in biological tissues. Very high peak intensities from focused short pulsed lasers are required for nonlinear excitation due to the extremely low probability of the simultaneous arrival of multiple photons of lower energy to excite fluorophores or interact with selective structures for harmonic generation. Combined with reduced scattering from the utilization of longer wavelengths, the inherent spatial confinement associated with achieving simultaneous arrival of photons within the focal volume enables deep imaging with low out-of-focus background for nonlinear imaging. This review provides an introduction to the different contrast mechanisms available with nonlinear imaging and instrumentation commonly used in nonlinear microscopy. Furthermore, we discuss some recent advances in nonlinear microscopy to extend the imaging penetration depth, conduct histopathological investigations on fresh tissues and examine the molecular order and orientation of molecules using polarization nonlinear microscopy.

Indexed as

PhotonsAnimalsFluorescenceHumansImage EnhancementLightingNonlinear Optical MicroscopyScattering, RadiationHarmonic generationImagingMultiphoton microscopyNonlinear microscopy

Identifiers

PMID33253831
PMCPMC8177044
OpenAlexW3106684002

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.