ArticleClinical infectious diseases : an official publication of the Infectious Diseases Society of America2021
AcanR3990 qPCR: A Novel, Highly Sensitive, Bioinformatically-Informed Assay to Detect Angiostrongylus cantonensis Infections.
Article in Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 50 citations in OpenAlex.
- Predictive Approach to Mapping Angiostrongylus cantonensis Nematode Distribution, Canary Islands, Spain.Emerging infectious diseases · 2026Article
- Sensitive Detection of Foodborne Parasitic Larvae Using a Sieving-qPCR Workflow: Application to Angiostrongylus cantonensis.Journal of food science · 2026Article
- Assessment of the Prevalence of Angiostrongylus cantonensis Infection in Gastropods of Puerto Rico.The American journal of tropical medicine and hygiene · 2026Article
- Development of Recombinase Polymerase Amplification and CRISPR-Cas12a-Enhanced Isothermal Amplification Assays for Strongyloides stercoralis DNA Detection: A Pilot Study.The American journal of tropical medicine and hygiene · 2026Article
- An Outbreak of Neuroangiostrongyliasis (Rat Lungworm Disease) in Hawai'i: Evidence Supporting the Early Use of Albendazole-Corticosteroid Co-Therapy.The American journal of tropical medicine and hygiene · 2026Article
- Development of a TaqMan qPCR Method for DetectingTropical medicine and infectious disease · 2026Article
- Hedgehogs and Angiostrongylus cantonensis: Uncovering the Role of Atelerix albiventris in the Parasite Life Cycle.Integrative zoology · 2026Article
- Central nervous system infection by Angiostrongylus cantonensis in children: experience from Guangdong, China.BMC infectious diseases · 2025Article
- Seroprevalence ofFood and waterborne parasitology · 2025Article
- Hidden threats in urban environments: Angiostrongylus cantonensis in Banda Aceh's cityscape.PLoS neglected tropical diseases · 2025Article
- Same parasite, diverging fates: distinct responses of tenrecs to Angiostrongylus cantonensis infection.Veterinary research communications · 2025Article
- Neuroangiostrongyliasis Infection Risk Near Preschool Centres in Mallorca, Spain: A Pilot Micro-Epidemiological Study.Zoonoses and public health · 2025Article
- Rainfall- and Temperature-Driven Emergence of Neural Angiostrongyliasis in Eastern Australia, 2020-2024.The Journal of infectious diseases · 2025Article
- Insights into the biology of the rat lungworm, Angiostrongylus cantonensis.Parasites & vectors · 2025Review
- A cost-effective strategy for identifying Angiostrongylus spp. larvae in Achatina fulica: combined morphological and molecular biology.Parasites & vectors · 2025Article
- Improved Nucleic Acid Amplification Test for the Diagnosis of Onchocerciasis and Its Use for Detection of Circulating Cell-free DNA.The Journal of infectious diseases · 2025Article
- Isolate-specific rat brain transcriptional responses to rat lungworm (Angiostrongylus cantonensis).Pathogens and disease · 2025Article
- Passage ofParasitology · 2025Article
- Lizards as sentinels for the distribution ofEpidemiology and infection · 2024Article
- Dysesthesias and migratory myalgias: clinical clues to suspect neuroangiostrongyliasis.Acta neurologica Belgica · 2024Article
Corrections and comments
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Authors and funding
11 authors at 7 institutions in 3 countries.
Funding
Abstract
backgroundAngiostrongylus cantonensis (Ac), or the rat lungworm, is a major cause of eosinophilic meningitis. Humans are infected by ingesting the 3rd stage larvae from primary hosts, snails, and slugs, or paratenic hosts. The currently used molecular test is a qPCR assay targeting the ITS1 rDNA region (ITS1) of Ac.
methodsIn silico design of a more sensitive qPCR assay was performed based on tandem repeats predicted to be the most abundant by the RepeatExplorer algorithm. Genomic DNA (gDNA) of Ac were used to determine the analytical sensitivity and specificity of the best primer/probe combination. This assay was then applied to clinical and environmental samples.
resultsThe limit of detection of the best performing assay, AcanR3990, was 1 fg (the DNA equivalent of 1/100 000 dilution of a single 3rd stage larvae). Out of 127 CDC archived CSF samples from varied geographic locations, the AcanR3990 qPCR detected the presence of Ac in 49/49 ITS1 confirmed angiostrongyliasis patients, along with 15/73 samples previously negative by ITS1 qPCR despite strong clinical suspicion for angiostrongyliasis. Intermediate hosts (gastropods) and an accidental host, a symptomatic horse, were also tested with similar improvement in detection observed. AcanR3990 qPCR did not cross-react in 5 CSF from patients with proven neurocysticercosis, toxocariasis, gnathostomiasis, and baylisascariasis. AcanR3990 qPCR failed to amplify genomic DNA from the other related Angiostrongylus species tested except for Angiostrongylus mackerrasae (Am), a neurotropic species limited to Australia that would be expected to present with a clinical syndrome indistinguishable from Ac.
conclusionThese results suggest AcanR3990 qPCR assay is highly sensitive and specific with potential wide applicability as a One Health detection method for Ac and Am.
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