ArticleFrontiers in bioengineering and biotechnology2020
A Custom Ultra-Low-Cost 3D Bioprinter Supports Cell Growth and Differentiation.
Article in Frontiers in bioengineering and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Strategies in Tissue Regenerative Engineering for the Treatment of Human Infertility.Stem cell reviews and reports · 2025Review
- Effects of Polyhydroxybutyrate-co-hydroxyvalerate Microparticle Loading on Rheology, Microstructure, and Processability of Hydrogel-Based Inks for Bioprinted and Moulded Scaffolds.Gels (Basel, Switzerland) · 2025Article
- Extrusion bioprinting: meeting the promise of human tissue biofabrication?Progress in biomedical engineering (Bristol, England) · 2025Review
- A Low-Cost, Open-Source 3D Printer for Multimaterial and High-Throughput Direct Ink Writing of Soft and Living Materials.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Injectable and 3D-Printable Semi-Interpenetrating Polymer Networks Based on Modified Sodium Alginate for Cell Spheroid Formation.Biomacromolecules · 2025Article
- The cutting-edge progress in bioprinting for biomedicine: principles, applications, and future perspectives.MedComm · 2024Review
- Photo-/thermo-responsive bioink for improved printability in extrusion-based bioprinting.Materials today. Bio · 2024Article
- The Enderstruder: An accessible open-source syringe extruder compatible with Ender series 3D printers.HardwareX · 2024Article
- Biomaterials for extrusion-based bioprinting and biomedical applications.Frontiers in bioengineering and biotechnology · 2024Review
- Fibrinogen-Based Bioink for Application in Skin Equivalent 3D Bioprinting.Journal of functional biomaterials · 2023Article
- Investigating multi-material hydrogel three-dimensional printing forRoyal Society open science · 2023Article
- The Variety of 3D Breast Cancer Models for the Study of Tumor Physiology and Drug Screening.International journal of molecular sciences · 2023Review
- Modeling a Dynamic Printability Window on Polysaccharide Blend Inks for Extrusion Bioprinting.ACS biomaterials science & engineering · 2023Article
- Development and implementation of a significantly low-cost 3D bioprinter using recycled scrap material.Frontiers in bioengineering and biotechnology · 2023Article
- Development of a high-performance open-source 3D bioprinter.Scientific reports · 2022Article
- Different Curcumin-Loaded Delivery Systems for Wound Healing Applications: A Comprehensive Review.Pharmaceutics · 2022Review
- Systematic review on the application of 3D-bioprinting technology in orthoregeneration: current achievements and open challenges.Journal of experimental orthopaedics · 2022Review
- Vascularization in Bioartificial Parenchymal Tissue: Bioink and Bioprinting Strategies.International journal of molecular sciences · 2022Review
- Designing Cost-Effective Open-Source Multihead 3D Bioprinters.GEN biotechnology · 2022Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advances in 3D bioprinting have allowed the use of stem cells along with biomaterials and growth factors toward novel tissue engineering approaches. However, the cost of these systems along with their consumables is currently extremely high, limiting their applicability. To address this, we converted a 3D printer into an open source 3D bioprinter and produced a customized bioink based on accessible alginate/gelatin precursors, leading to a cost-effective solution. The bioprinter's resolution, including line width, spreading ratio and extrusion uniformity measurements, along with the rheological properties of the bioinks were analyzed, revealing high bioprinting accuracy within the printability window. Following the bioprinting process, cell survival and proliferation were validated on HeLa Kyoto and HEK293T cell lines. In addition, we isolated and 3D bioprinted postnatal neural stem cell progenitors derived from the mouse subventricular zone as well as mesenchymal stem cells derived from mouse bone marrow. Our results suggest that our low-cost 3D bioprinter can support cell proliferation and differentiation of two different types of primary stem cell populations, indicating that it can be used as a reliable tool for developing efficient research models for stem cell research and tissue engineering.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.