ArticlePoultry science2020
Rapid on-site detection of Salmonella pullorum based on lateral flow nucleic acid assay combined with recombinase polymerase amplification reaction.
Article in Poultry science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 19 citations in OpenAlex.
- Recent advances in isothermal amplification techniques for detection of animal diseases.Frontiers in veterinary science · 2026Review
- Establishment and application of a dual RPA-LFD rapid detection method for Salmonella Pullorum and Salmonella Enteritidis.PloS one · 2025Article
- Optimized detection of Salmonella typhimurium using aptamer lateral flow assay.Biotechnology letters · 2024Article
- Rapid and specific detection of Enterococcus faecium with an isothermal amplification and lateral flow strip combined method.Archives of microbiology · 2023Article
- Research Progress of Nucleic Acid Detection Technology for Genetically Modified Maize.International journal of molecular sciences · 2023Review
- Development and Application of Real-Time PCR Assay for Detection of Salmonella Abortusequi.Journal of clinical microbiology · 2023Article
- Protective effect and possible mechanism of arctiin on broilers challenged by Salmonella pullorum.Journal of animal science · 2022Article
- An Enhanced Lateral Flow Assay Based on Aptamer-Magnetic Separation and Multifold AuNPs for Ultrasensitive Detection ofFoods (Basel, Switzerland) · 2021Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Caused by Salmonella pullorum, pullorosis is a bacterial disease threatening the poultry industry and has been listed as the bacterial disease to be eliminated by the government. However, antibiotic treatment of pullorosis has become increasingly difficult, resulting in severe influences on the sustainable development of poultry. Abuse of antibiotics may cause global drug-resistant problems. Hence, early diagnosis of young chickens and accurate treatment of sick chickens are urgently needed. Traditional serotyping for Salmonella detection is costly and labor-intensive, whereas other commonly used plate agglutination test methods often cause physical injury of chickens. Therefore, a rapid and nondamaging detection method is of great significance for early diagnosis, which is the key step in accurate medication and elimination of pullorosis. In this study, we propose a novel lateral flow nucleic acid assay (LFNAA) system combining recombinase polymerase amplification (RPA) for the detection of S. pullorum. In this method, the DNA of S. pullorum strains was quickly amplified by RPA under 37°C, and then, the RPA products were added onto the LFNAA sample pad until the final results could be observed by naked eyes within 3 min. The proposed assay is fast and delivers visible results to naked eyes in field test. The limit of detection for genomic DNA was 5 × 10
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