ArticleMolecular metabolism2021
Adaptive thermogenesis in brown adipose tissue involves activation of pannexin-1 channels.
Article in Molecular metabolism, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- ATP Release and Purinergic Signaling Potentiate β-Adrenergic Effects on Brown Adipocytes.Acta physiologica (Oxford, England) · 2026Article
- Panx1 deficiency exacerbates GAN diet-induced obesity by destabilizing β-catenin via GSK3β.iScience · 2026Article
- Energy's long game: thermogenic adipocytes and the metabolic diseases of modern life.Academia biology · 2026Article
- Purinergic adipocyte-macrophage crosstalk promotes degeneration of thermogenic brown adipose tissue.EMBO reports · 2025Article
- Brown and beige adipose tissue-derived metabokine and lipokine inter-organ signalling in health and disease.Experimental physiology · 2025Review
- An efficient AAV vector system of Rec2 serotype for intravenous injection to study metabolism in brown adipocytes in vivo.Molecular metabolism · 2024Article
- Cold Exposure Rejuvenates the Metabolic Phenotype ofBiomolecules · 2024Article
- Cardiomyocyte PANX1 Controls Glycolysis and Neutrophil Recruitment in Hypertrophy.Circulation research · 2024Article
- PANX1-mediated ATP release confers FAM3A's suppression effects on hepatic gluconeogenesis and lipogenesis.Military Medical Research · 2024Article
- The molecular mechanism of macrophage-adipocyte crosstalk in maintaining energy homeostasis.Frontiers in immunology · 2024Review
- Article
- Measurement of Intracellular Temperature in Brown Adipocytes Using a Cationic Fluorescent Polymeric Thermometer.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Brown adipose tissue influences adiponectin and thyroid hormone changes during Graves' disease therapy.Adipocyte · 2022Article
- Substantial impact of 3-iodothyronamine (T1AM) on the regulations of fluorescent thermoprobe-measured cellular temperature and natriuretic peptide expression in cardiomyocytes.Scientific reports · 2022Article
- Pannexin 3 deletion reduces fat accumulation and inflammation in a sex-specific manner.International journal of obesity (2005) · 2022Article
- Intracellular thermometry uncovers spontaneous thermogenesis and associated thermal signaling.Communications biology · 2021Review
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Authors and funding
21 authors at 5 institutions in 2 countries.
Funding
Abstract
objectiveBrown adipose tissue (BAT) is specialized in thermogenesis. The conversion of energy into heat in brown adipocytes proceeds via stimulation of β-adrenergic receptor (βAR)-dependent signaling and activation of mitochondrial uncoupling protein 1 (UCP1). We have previously demonstrated a functional role for pannexin-1 (Panx1) channels in white adipose tissue; however, it is not known whether Panx1 channels play a role in the regulation of brown adipocyte function. Here, we tested the hypothesis that Panx1 channels are involved in brown adipocyte activation and thermogenesis.
methodsIn an immortalized brown pre-adipocytes cell line, Panx1 currents were measured using patch-clamp electrophysiology. Flow cytometry was used for assessment of dye uptake and luminescence assays for adenosine triphosphate (ATP) release, and cellular temperature measurement was performed using a ratiometric fluorescence thermometer. We used RNA interference and expression plasmids to manipulate expression of wild-type and mutant Panx1. We used previously described adipocyte-specific Panx1 knockout mice (Panx1
resultsIn brown adipocytes, Panx1 channel activity was induced either by apoptosis-dependent caspase activation or by β3AR stimulation via a novel mechanism that involves Gβγ subunit binding to Panx1. Inactivation of Panx1 channels in cultured brown adipocytes resulted in inhibition of β3AR-induced lipolysis, UCP-1 expression, and cellular thermogenesis. In mice, adiponectin-Cre-dependent genetic deletion of Panx1 in all adipose tissue depots resulted in defective β3AR agonist- or cold-induced thermogenesis in BAT and suppressed beigeing of white adipose tissue. UCP1-Cre-dependent Panx1 deletion specifically in brown adipocytes reduced the capacity for adaptive thermogenesis without affecting beigeing of white adipose tissue and aggravated diet-induced obesity and insulin resistance.
conclusionsThese data demonstrate that Gβγ-dependent Panx1 channel activation is involved in β3AR-induced thermogenic regulation in brown adipocytes. Identification of Panx1 channels in BAT as novel thermo-regulatory elements downstream of β3AR activation may have therapeutic implications.
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