ArticleThe Laryngoscope2021
Laryngeal Reconstruction Using Tissue-Engineered Implants in Pigs: A Pilot Study.
Article in The Laryngoscope, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Vocal Outcomes After Cell-Based Outer Vocal Fold Implant in Porcine Bilateral Vocal Fold Resection.The Laryngoscope · 2026Article
- Immunotolerant Oligomer scaffolds promote regenerative remodeling and improved muscle structure and function after volumetric muscle loss.Scientific reports · 2026Article
- Novel Biomolecule-Infused Gelatin Injectable for Treatment of Recurrent Laryngeal Nerve Injury.The Laryngoscope · 2025Article
- Multi-Layered Implant Approach for Hemilaryngectomy Reconstruction in a Porcine Model.The Laryngoscope · 2025Article
- Optimizing transport methods to preserve function of self-innervating muscle cells for laryngeal injection.Laryngoscope investigative otolaryngology · 2024Article
- Tissue-Engineered Implant for Hemilaryngectomy Reconstruction with Recurrent Laryngeal Nerve Injury.The Laryngoscope · 2024Article
- Effect of amniotic membrane/collagen scaffolds on laryngeal cartilage repair.Laryngoscope investigative otolaryngology · 2024Article
- Early Changes in Porcine Larynges Following Injection of Motor-Endplate Expressing Muscle Cells for the Treatment of Unilateral Vocal Fold Paralysis.The Laryngoscope · 2024Article
- Engineered collagen polymeric materials create noninflammatory regenerative microenvironments that avoid classical foreign body responses.Biomaterials science · 2023Article
- Tissue-engineered vocal fold replacement in swine: Methods for functional and structural analysis.PloS one · 2023Article
- Impact of Needle Selection on Survival of Muscle-Derived Cells When Used for Laryngeal Injections.Journal of cell science & therapy · 2023Article
- Mechanobiological wound model for improved design and evaluation of collagen dermal replacement scaffolds.Acta biomaterialia · 2021Article
- Objective Assessment of Porcine Voice Acoustics for Laryngeal Surgical Modeling.Applied sciences (Basel, Switzerland) · 2021Article
- Regenerative tissue filler for breast conserving surgery and other soft tissue restoration and reconstruction needs.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
objective/hypothesisThere are currently no treatments available that restore dynamic laryngeal function after hemilaryngectomy. We have shown that dynamic function can be restored post hemilaryngectomy in a rat model. Here, we report in a first of its kind, proof of concept study that this previously published technique is scalable to a porcine model. STUDY
designAnimal study.
methodsMuscle and fat biopsies were taken from three Yucatan minipigs. Muscle progenitor cells (MPCs) and adipose stem cells (ASCs) were isolated and cultured for 3 weeks. The minipigs underwent a left laterovertical partial laryngectomy sparing the left arytenoid cartilage and transecting the recurrent laryngeal nerve. Each layer was replaced with a tissue-engineered implant: 1) an acellular mucosal layer composed of densified Type I oligomeric collagen, 2) a skeletal muscle layer composed of autologous MPCs and aligned oligomeric collagen differentiated and induced to express motor endplates (MEE), and 3) a cartilage layer composed of autologous ASCs and densified oligomeric collagen differentiated to cartilage. Healing was monitored at 2 and 4 weeks post-op, and at the 8 week study endpoint.
resultsAnimals demonstrated appropriate weight gain, no aspiration events, and audible phonation. Video laryngoscopy showed progressive healing with vascularization and re-epithelialization present at 4 weeks. On histology, there was no immune reaction to the implants and there was complete integration into host tissue with nerve and vascular ingrowth.
conclusionsThis pilot study represents a first in which a transmural vertical partial laryngectomy was performed and successfully repaired with a customized, autologous stem cell-derived multi-layered tissue-engineered implant. LEVEL OF EVIDENCE: NA Laryngoscope, 131:2277-2284, 2021.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.