ArticleCommunications biology2020
Ligand-directed covalent labelling of a GPCR with a fluorescent tag in live cells.
Article in Communications biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Progress and challenges in traceless ligand-directed labelling chemistry.Communications chemistry · 2026Review
- Two-Step Ligand-Directed Covalent Fluorescent Labeling of the Adenosine AJournal of medicinal chemistry · 2026Article
- pH sensing and inflammation: linking acidic microenvironments to immune reprogramming.Frontiers in immunology · 2026Review
- Dual blockade of adenosine AFrontiers in immunology · 2026Article
- Biocompatible sulfonium-based covalent probes for endogenous tubulin fluorescence nanoscopy in live and fixed cells.Nature communications · 2025Article
- Bitopic AJournal of medicinal chemistry · 2025Article
- Ligand-directed covalent labelling of adenosine receptors.Purinergic signalling · 2025Article
- Chemical biology approaches to resolve the subcellular GPCR signaling landscape.Nature chemical biology · 2025Review
- A modular click ligand-directed approach to label endogenous dopamine DCommunications chemistry · 2025Article
- Design, Synthesis, and Evaluation of a New Fluorescent Ligand for the MACS medicinal chemistry letters · 2025Article
- Covalent functionalization of G protein-coupled receptors by small molecular probes.RSC chemical biology · 2025Review
- Lipid-Directed Covalent Labeling of Plasma Membranes for Long-Term Imaging, Barcoding and Manipulation of Cells.JACS Au · 2025Article
- Photoproximity labeling of endogenous receptors in the live mouse brain in minutes.Nature chemical biology · 2025Article
- A novel and selective fluorescent ligand for the study of adenosine APharmacology research & perspectives · 2024Article
- Ligand-Directed Labeling of the Adenosine AJournal of medicinal chemistry · 2024Article
- Article
- Chemical biology tools for protein labelling: insights into cell-cell communication.The Biochemical journal · 2023Review
- Fluorescent APurinergic signalling · 2023Article
- Expanding the apelin receptor pharmacological toolbox using novel fluorescent ligands.Frontiers in endocrinology · 2023Article
- Detecting and measuring of GPCR signaling - comparison of human induced pluripotent stem cells and immortal cell lines.Frontiers in endocrinology · 2023Review
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
To study the localisation of G protein-coupled receptors (GPCR) in their native cellular environment requires their visualisation through fluorescent labelling. To overcome the requirement for genetic modification of the receptor or the limitations of dissociable fluorescent ligands, here we describe rational design of a compound that covalently and selectively labels a GPCR in living cells with a fluorescent moiety. We designed a fluorescent antagonist, in which the linker incorporated between pharmacophore (ZM241385) and fluorophore (sulfo-cyanine5) is able to facilitate covalent linking of the fluorophore to the adenosine A
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.