ArticleNature communications2020
High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
What it found
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Who cites it
66 citing papers in PubMed, 126 citations in OpenAlex.
- Two-photon light-sheet microscopy with isotropic near-diffraction-limited resolution in three dimensions.iScience · 2026Article
- Preclinical in-vivo cerebrovascular imaging in rodent models: Methods and applications in Alzheimer's disease.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2026Review
- Integrated two-photon and photoacoustic microscopy for single-cell neurometabolic imaging.Nature communications · 2026Article
- PAVSAT: an automated blood vessel analysis tool using deep learning-based segmentation and image processing.BMC bioinformatics · 2026Article
- Tunable Bessel beam two-photon fluorescence microscopy for high-speed volumetric imaging of brain dynamics.eLife · 2026Article
- Blood-brain barrier breakdown in traumatic brain injury: current insights and future directions.Cancer gene therapy · 2026Review
- A robotic patch-clamp system with real-time localization and phase-synchronized capture of dynamic in vivo cells using micropipette resistance modelling.Microsystems & nanoengineering · 2026Article
- Improving positively tuned voltage indicators for faster kinetics and higher contrast.bioRxiv : the preprint server for biology · 2026Article
- Cerebral capillary computation.American journal of physiology. Cell physiology · 2026Review
- Multi-parametric longitudinal imaging of cerebral biomarkers in a rodent model of Alzheimer's disease.Photoacoustics · 2026Article
- Super-resolution functional photoacoustic microscopy via label-free cell tracking.Light, science & applications · 2026Article
- Bessel beam side lobe suppression via non-degenerate two-photon excitation.Biomedical optics express · 2026Article
- Correlative Imaging Platform Linking Taste Cell Function to Molecular Identity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Tunable Bessel beam two-photon fluorescence microscopy for high-speed volumetric imaging of brain dynamics.bioRxiv : the preprint server for biology · 2026Article
- Needle beam two-photon microscopy for simultaneous multiplane neural and vascular imaging in awake mice.PhotoniX · 2026Article
- Metalens for passive generation of a droplet Bessel beam.Microsystems & nanoengineering · 2025Article
- Compact way to generate guide star for wavefront sensing in Bessel two-photon microscopy.Scientific reports · 2025Article
- Dual-Channel Event Microscopy for Ultrafast Biological Imaging.bioRxiv : the preprint server for biology · 2025Article
- Descattering and image restoration with a transformer-based neural network in deep tissue imaging.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A cortex-wide multimodal microscope for simultaneous CaNature communications · 2025Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 5 institutions in 1 country.
Funding
Abstract
Understanding the structure and function of vasculature in the brain requires us to monitor distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) volumes in vivo. Currently, a volumetric vasculature imaging method with sub-capillary spatial resolution and blood flow-resolving speed is lacking. Here, using two-photon laser scanning microscopy (TPLSM) with an axially extended Bessel focus, we capture volumetric hemodynamics in the awake mouse brain at a spatiotemporal resolution sufficient for measuring capillary size and blood flow. With Bessel TPLSM, the fluorescence signal of a vessel becomes proportional to its size, which enables convenient intensity-based analysis of vessel dilation and constriction dynamics in large volumes. We observe entrainment of vasodilation and vasoconstriction with pupil diameter and measure 3D blood flow at 99 volumes/second. Demonstrating high-throughput monitoring of hemodynamics in the awake brain, we expect Bessel TPLSM to make broad impacts on neurovasculature research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.