ArticleMetabolic engineering2021
Metabolic engineering of tomato fruit enriched in L-DOPA.
Article in Metabolic engineering, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Heterologous Production of Forskolin in Tobacco (Nicotiana tabacum) via Glandular Trichome Specific Engineering and Metabolic Flux Redirection.Plant biotechnology journal · 2026Article
- Neuronutrition and Nrf2 Brain Resilience Signaling: Epigenomics and Metabolomics for Personalized Medicine in Nervous System Disorders from Bench to Clinic.International journal of molecular sciences · 2025Review
- Optimizing a tomato crocin biofactory by fine-tuning plant architecture.Frontiers in plant science · 2025Article
- Combined metabolome and transcriptome analysis reveal the mechanism of water stress in Ophiocordyceps sinensis.BMC genomics · 2024Article
- Improvement and application of vacuum-infiltration system in tomato.Horticulture research · 2024Article
- Spatio-temporal expression of polyphenol oxidase unveils the dynamics of L-DOPA accumulation in faba bean (Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Metabolomics to understand metabolic regulation underpinning fruit ripening, development, and quality.Journal of experimental botany · 2024Review
- SlERF.G3-Like mediates a hierarchical transcriptional cascade to regulate ripening and metabolic changes in tomato fruit.Plant biotechnology journal · 2024Article
- From qualitative to quantitative: the state of the art and challenges for plant synthetic biology.Quantitative biology (Beijing, China) · 2023Review
- Heterologous mogrosides biosynthesis in cucumber and tomato by genetic manipulation.Communications biology · 2023Article
- Effects of Chilling Treatment on Baicalin, Baicalein, and Wogonin Biosynthesis inPlants (Basel, Switzerland) · 2022Article
- Spatially resolved metabolomics reveals variety-specific metabolic changes in banana pulp during postharvest senescence.Food chemistry: X · 2022Article
- Plant Metabolic Engineering by Multigene Stacking: Synthesis of Diverse Mogrosides.International journal of molecular sciences · 2022Article
- Heterologous Biosynthesis of Health-Promoting Baicalein inMolecules (Basel, Switzerland) · 2022Article
- Combinatorial transient gene expression strategies to enhance terpenoid production in plants.Frontiers in plant science · 2022Article
- Benchmarking Intrinsic Promoters and Terminators for Plant Synthetic Biology Research.Biodesign research · 2022Article
- Diverting tyrosine: Data from untargeted metabolic analysis of tomato fruit accumulating L-DOPA.Data in brief · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
L-DOPA, also known as Levodopa or L-3,4-dihydroxyphenylalanine, is a non-standard amino acid, and the gold standard drug for the treatment for Parkinson's Disease (PD). Recently, a gene encoding the enzyme that is responsible for its synthesis, as a precursor of the coloured pigment group betalains, was identified in beetroot, BvCYP76AD6. We have engineered tomato fruit enriched in L-DOPA through overexpression of BvCYP76AD6 in a fruit specific manner. Analysis of the transgenic fruit revealed the feasibility of accumulating L-DOPA in a non-naturally betalain-producing plant. Fruit accumulating L-DOPA also showed major effects on the fruit metabolome. Some of these changes included elevation of amino acids levels, changes in the levels of intermediates of the TCA and glycolysis pathways and reductions in the levels of phenolic compounds and nitrogen-containing specialised metabolites. Furthermore, we were able to increase the L-DOPA levels further by elevating the expression of the metabolic master regulator, MYB12, specifically in tomato fruit, together with BvCYP76AD6. Our study elucidated new roles for L-DOPA in plants, because it impacted fruit quality parameters including antioxidant capacity and firmness. The L-DOPA levels achieved in tomato fruit were comparable to the levels in other non-seed organs of L-DOPA - accumulating plants, offering an opportunity to develop new biological sources of L-DOPA by widening the repertoire of L-DOPA-accumulating plants. These tomato fruit could be used as an alternative source of this important pharmaceutical.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.