ArticleJournal of neuroscience methods2021
Compensation of physiological motion enables high-yield whole-cell recording in vivo.
Article in Journal of neuroscience methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed.
- A robotic patch-clamp system with real-time localization and phase-synchronized capture of dynamic in vivo cells using micropipette resistance modelling.Microsystems & nanoengineering · 2026Article
- Method for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts.Bio-protocol · 2021Article
- Rapid Cortical Adaptation and the Role of Thalamic Synchrony during Wakefulness.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
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7 authors.
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Abstract
backgroundWhole-cell patch-clamp recording in vivo is the gold-standard method for measuring subthreshold electrophysiology from single cells during behavioural tasks, sensory stimulations, and optogenetic manipulation. However, these recordings require a tight, gigaohm resistance, seal between a glass pipette electrode's aperture and a cell's membrane. These seals are difficult to form, especially in vivo, in part because of a strong dependence on the distance between the pipette aperture and cell membrane. NEW
methodWe elucidate and utilize this dependency to develop an autonomous method for placement and synchronization of pipette's tip aperture to the membrane of a nearby, moving neuron, which enables high-yield seal formation and subsequent recordings deep in the brain of the living mouse.
resultsThis synchronization procedure nearly doubles the reported gigaseal yield in the thalamus (>3 mm below the pial surface) from 26 % (n = 17/64) to 48 % (n = 32/66). Whole-cell recording yield improved from 10 % (n = 9/88) to 24 % (n = 18/76) when motion compensation was used during the gigaseal formation. As an example of its application, we utilized this system to investigate the role of the sensory environment and ventral posterior medial region (VPM) projection synchrony on intracellular dynamics in the barrel cortex. COMPARISON WITH EXISTING METHOD(S): Current methods of in vivo whole-cell patch clamping do not synchronize the position of the pipette to motion of the cell.
conclusionsThis method results in substantially greater subcortical whole-cell recording yield than previously reported and thus makes pan-brain whole-cell electrophysiology practical in the living mouse brain.
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