Evidence map›Paper›PMID 33242491›Full record

ArticleExperimental eye research2021

Retinal safety evaluation of photoacoustic microscopy.

Yanxiu Li, Wei Zhang, Van Phuc Nguyen, Naheed Wali Khan, Xiaobo Xia, Xueding Wang, Yannis M Paulus

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental eye research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
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  4. Molecular and cellular imaging of the eye.Biomedical optics express · 2024
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Yanxiu LiUniversity of Michigan, Department of Ophthalmology and Visual Sciences, 1000 Wall Street, Ann Arbor, MI, 48105, United States; Central South University, Eye Center of Xiangya Hospital, NO. 87 Xiangya Road, Kaifu District, Changsha, Hunan, 410008, PR China; Central South University, Hunan Key Laboratory of Ophthalmology, NO. 87 Xiangya Road, Kaifu District, Changsha, Hunan, 410008, PR China. Electronic address: yanxiuli@umich.edu.
Wei ZhangUniversity of Michigan, Department of Biomedical Engineering, 2200 Bonisteel Blvd, Ann Arbor, MI, 48109, United States. Electronic address: zhawei@umich.edu.
Van Phuc NguyenUniversity of Michigan, Department of Ophthalmology and Visual Sciences, 1000 Wall Street, Ann Arbor, MI, 48105, United States. Electronic address: vanphucn@umich.edu.
Naheed Wali KhanUniversity of Michigan, Department of Ophthalmology and Visual Sciences, 1000 Wall Street, Ann Arbor, MI, 48105, United States. Electronic address: nwkhan@med.umich.edu.
Xiaobo XiaCentral South University, Eye Center of Xiangya Hospital, NO. 87 Xiangya Road, Kaifu District, Changsha, Hunan, 410008, PR China; Central South University, Hunan Key Laboratory of Ophthalmology, NO. 87 Xiangya Road, Kaifu District, Changsha, Hunan, 410008, PR China. Electronic address: xbxia21@163.com.
Xueding WangUniversity of Michigan, Department of Biomedical Engineering, 2200 Bonisteel Blvd, Ann Arbor, MI, 48109, United States. Electronic address: xdwang@umich.edu.
Yannis M PaulusUniversity of Michigan, Department of Ophthalmology and Visual Sciences, 1000 Wall Street, Ann Arbor, MI, 48105, United States; University of Michigan, Department of Biomedical Engineering, 2200 Bonisteel Blvd, Ann Arbor, MI, 48109, United States. Electronic address: ypaulus@med.umich.edu.
University of Michigan · USCentral South University · CN

Funding

VIVARIUM MODULEP30EY007003 · NEI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI David Antonetti · 1987 to 2026
$17.6M
Real-time In Vivo Visualization of the Molecular Processes in Choroidal NeovascularizationK08EY027458 · NEI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI PAULUS, YANNIS MANTAS · 2018 to 2021
$898k
NEI NIH HHS K08 EY027458NEI NIH HHS P30 EY007003
6 · The paper itself

Abstract

Photoacoustic microscopy (PAM) has significant potential as a promising diagnostic method for eye diseases and can provide anatomic and functional information of the retinal and choroidal vasculature. However, there are no FDA-approved PAM systems for ophthalmic imaging. In this study, a comprehensive safety evaluation was performed to evaluate the safety of PAM retinal imaging and whether PAM causes damage to retinal structure or function in rabbit eyes. 12 Dutch-Belted pigmented rabbits received photoacoustic imaging to 57% of the retinal surface area with a laser energy of 5% of the ANSI safety limit for five consecutive days and followed before imaging and 3 days, 1, 2, 3, and 4 weeks post imaging. Retinal morphologic analyses using slit lamp examination, fundus photography, red free, FA, FAF, ICGA, and OCT showed no retinal hemorrhage, edema, detachment, vascular abnormalities, or pigmentary abnormalities in the retina or choroid after PAM imaging. Full-field ERG analysis showed no significant difference in scotopic or photopic a- and b-wave amplitudes or implicit times between the control and experimental eyes over time (n = 6, P values > 0.05). Retinal ultrastructural evaluation using TEM showed normal structure of organelles and nuclei, and no significant loss of cells after PAM. TUNEL assay showed no evidence of cells apoptosis in retina. Retinal histopathology indicated that the architecture and thickness of the retinal layers was well preserved in all experimental eyes. A positive control at 500% of the ANSI limit demonstrated significant damage. The comprehensive retinal safety evaluation demonstrated no damage to retinal structure or function for 4 weeks after PAM imaging in rabbits.

Indexed as

AnimalsMicroscopy, AcousticModels, AnimalPhotoacoustic TechniquesRabbitsReproducibility of ResultsRetinaRetinal VesselsERGPhotoacoustic microscopyRetinal imagingSafety evaluation

Identifiers

PMID33242491
PMCPMC8018531
OpenAlexW3107324577

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.