Evidence map›Paper›PMID 33241692›Full record

ArticleBioconjugate chemistry2021

Optimal His-Tag Design for Efficient [

Jennifer D Williams, Florian Kampmeier, Adam Badar, Kevin Howland, Margaret S Cooper, Gregory E D Mullen, Philip J Blower

Open access · greenAbstract read
In one paragraph

Article in Bioconjugate chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.8field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 14 citations in OpenAlex.

  1. From [Molecules (Basel, Switzerland) · 2026
    Article
  2. Review
  3. Review
  4. Preclinical development and characterisation ofAmerican journal of nuclear medicine and molecular imaging · 2021
    Article
  5. RadioLabeling of the Mutant form of Anti-PlGF Nanobody byIranian journal of pharmaceutical research : IJPR
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Jennifer D WilliamsKing's College London, School of Biomedical Engineering and Imaging Sciences, 4th Floor Lambeth Wing, St Thomas' Hospital, London SE1 7EH, United Kingdom.
Florian KampmeierKing's College London, School of Biomedical Engineering and Imaging Sciences, 4th Floor Lambeth Wing, St Thomas' Hospital, London SE1 7EH, United Kingdom.
Adam BadarKing's College London, School of Biomedical Engineering and Imaging Sciences, 4th Floor Lambeth Wing, St Thomas' Hospital, London SE1 7EH, United Kingdom.
Kevin HowlandBiomolecular Science Facility, University of Kent, Canterbury, CT2 7NJ, United Kingdom.
Margaret S CooperKing's College London, School of Biomedical Engineering and Imaging Sciences, 4th Floor Lambeth Wing, St Thomas' Hospital, London SE1 7EH, United Kingdom.
Gregory E D MullenKing's College London, School of Biomedical Engineering and Imaging Sciences, 4th Floor Lambeth Wing, St Thomas' Hospital, London SE1 7EH, United Kingdom.
Philip J BlowerKing's College London, School of Biomedical Engineering and Imaging Sciences, 4th Floor Lambeth Wing, St Thomas' Hospital, London SE1 7EH, United Kingdom.ORCID 0000-0001-6290-1590
St Thomas' Hospital · GBUniversity of Kent · GB

Funding

Wellcome Trust 088641
6 · The paper itself

Abstract

Hexahistidine tags (His-tags), incorporated into recombinant proteins to facilitate purification using metal-affinity chromatography, are useful binding sites for radiolabeling with [

Indexed as

HistidineOrganotechnetium CompoundsPeptidesProteinsRadiopharmaceuticalsRheniumHistidineOrganotechnetium CompoundsPeptidesProteinsRadiopharmaceuticalsRhenium

Identifiers

PMID33241692
PMCPMC7611356
OpenAlexW3110286624

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.