ReviewSmall (Weinheim an der Bergstrasse, Germany)2020
High Throughput and Highly Controllable Methods for In Vitro Intracellular Delivery.
Review in Small (Weinheim an der Bergstrasse, Germany), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Reversibly-sealable microfluidic platform for multi-molecule gradient delivery to large adherent cell cultures.Biomedical microdevices · 2026Article
- A critical review of microfluidic electroporation for therapeutic cell engineering.Cell reports. Physical science · 2026Article
- Spatially-Resolved Organoid Transfection by Porous Silicon-Mediated Optoporation.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Quantitative analysis of electroporation-mediated intracellular delivery via bioorthogonal luminescent reaction.Communications chemistry · 2024Article
- HiViPore: a highly viable in-flow compression for a one-step cell mechanoporation in microfluidics to induce a free delivery of nano- macro-cargoes.Journal of nanobiotechnology · 2024Article
- Transepithelial Electrical Impedance Increase Following Porous Substrate Electroporation Enables Label-Free Delivery.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- High-throughput cell optoporation system based on Au nanoparticle layers mediated by resonant irradiation for precise and controllable gene delivery.Scientific reports · 2024Article
- CRISPR/Cas-Assisted Nanoneedle Sensor for Adenosine Triphosphate Detection in Living Cells.ACS applied materials & interfaces · 2023Article
- Continuous microfluidic flow-through protocol for selective and image-activated electroporation of single cells.RSC advances · 2023Article
- Single cell transcriptomics reveals reduced stress response in stem cells manipulated using localized electric fields.Materials today. Bio · 2023Article
- Multiplexed high-throughput localized electroporation workflow with deep learning-based analysis for cell engineering.Science advances · 2022Article
- Recent Advances in Microscale Electroporation.Chemical reviews · 2022Review
- Deep Learning-Assisted Automated Single Cell Electroporation Platform for Effective Genetic Manipulation of Hard-to-Transfect Cells.Small (Weinheim an der Bergstrasse, Germany) · 2022Article
- In Situ Electroporation on PERFECT Filter for High-Efficiency and High-Viability Tumor Cell Labeling.Micromachines · 2022Article
- An equivalent circuit model for localized electroporation on porous substrates.Biosensors & bioelectronics · 2022Article
- High-Throughput and Dosage-Controlled Intracellular Delivery of Large Cargos by an Acoustic-Electric Micro-Vortices Platform.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Microfluidic mechanoporation for cellular delivery and analysis.Materials today. Bio · 2022Review
- Sonoporation: Past, Present, and Future.Advanced materials technologies · 2022Article
- High-Efficient and Dosage-Controllable Intracellular Cargo Delivery through Electrochemical Metal-Organic Hybrid Nanogates.Small science · 2021Article
- Tutorial: using nanoneedles for intracellular delivery.Nature protocols · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
In vitro and ex vivo intracellular delivery methods hold the key for releasing the full potential of tissue engineering, drug development, and many other applications. In recent years, there has been significant progress in the design and implementation of intracellular delivery systems capable of delivery at the same scale as viral transfection and bulk electroporation but offering fewer adverse outcomes. This review strives to examine a variety of methods for in vitro and ex vivo intracellular delivery such as flow-through microfluidics, engineered substrates, and automated probe-based systems from the perspective of throughput and control. Special attention is paid to a particularly promising method of electroporation using micro/nanochannel based porous substrates, which expose small patches of cell membrane to permeabilizing electric field. Porous substrate electroporation parameters discussed include system design, cells and cargos used, transfection efficiency and cell viability, and the electric field and its effects on molecular transport. The review concludes with discussion of potential new innovations which can arise from specific aspects of porous substrate-based electroporation platforms and high throughput, high control methods in general.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.