Evidence map›Paper›PMID 33240823›Full record

ArticleFrontiers in cellular and infection microbiology2020

Cryofixation of Inactivated Hantavirus-Infected Cells as a Method for Obtaining High-Quality Ultrastructural Preservation for Electron Microscopic Studies.

Amar Parvate, Ranjan Sengupta, Evan P Williams, Yi Xue, Yong-Kyu Chu, Robert V Stahelin, Colleen B Jonsson

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Amar ParvateDepartment of Biological Sciences, Purdue University, West Lafayette, IN, United States.
Ranjan SenguptaDepartment of Biological Sciences, Purdue University, West Lafayette, IN, United States.
Evan P WilliamsDepartment of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN, United States.
Yi XueDepartment of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN, United States.
Yong-Kyu ChuCenter for Predictive Medicine, University of Louisville, Louisville, KY, United States.
Robert V StahelinMedicinal Chemistry and Molecular Pharmacology and the Purdue Institute for Inflammation, Immunology and Infectious Disease, Purdue University, West Lafayette, IN, United States.
Colleen B JonssonDepartment of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN, United States.
Purdue University West Lafayette · USUniversity of Tennessee Health Science Center · USUniversity of Louisville · US

Funding

LIPID-PROTEIN INTERACTIONS IN VIRAL ASSEMBLY AND VIRUS LIKE PARTICLE FORMATIONR01AI081077 · NIAID · PURDUE UNIVERSITY · PI STAHELIN, ROBERT VIRGIL · 2009 to 2019
$3.5M
Evolutonary Mechanisms of RNA Virus Host SwitchingR01AI103053 · NIAID · UNIVERSITY OF TENNESSEE KNOXVILLE · PI JONSSON, COLLEEN B · 2014 to 2017
$1.7M
NIAID NIH HHS R01 AI081077NIAID NIH HHS R01 AI103053
6 · The paper itself

Abstract

Hantaviruses rewire the host cell and induce extensive membrane rearrangements for their replication and the morphogenesis of the virion. Transmission electron microscopy (TEM) is a powerful technique for imaging these pathological membrane changes especially when combined with large volume electron tomography. Excellent preservation of membrane structure can be obtained when chemical fixation is combined with cryofixation via high pressure freezing making the samples amenable to serial-section tomographic reconstruction. Taking advantage of this, we have optimized a hybrid method that employs aldehyde fixation, a step that is essential for virus inactivation, followed by high-pressure freezing for ultrastructural study of Hantaan (HTN) and Andes (AND) virus infected Vero E6 cells. HTNV and ANDV are two species of the

Indexed as

Hantavirus InfectionsHantavirus Pulmonary SyndromeOrthohantavirusAnimalsChlorocebus aethiopsCryopreservationElectronsHumansVero CellsAndes viruscryofixationHantaan virushantavirusesHPS-FSmembrane morphologyTEMvirus-inactivation

Identifiers

PMID33240823
PMCPMC7677528
OpenAlexW3097278217

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.