ArticleParasites & vectors2020
Use of Oxford Nanopore MinION to generate full-length sequences of the Blastocystis small subunit (SSU) rRNA gene.
Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Aspects of Genetic Diversity, Host Specificity and Public Health Significance of Single-Celled Intestinal Parasites Commonly Observed in Humans and Mostly Referred to as 'Non-Pathogenic'.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2025Review
- Blastocystis in free-ranging wild ruminant species across the Iberian Peninsula.Veterinary research · 2025Article
- Long-read metagenomic sequencing negates inferred loss of cytosine methylation in Myxosporea (Cnidaria: Myxozoa).GigaScience · 2025Article
- A cross-sectional survey ofOne health (Amsterdam, Netherlands) · 2024Article
- New Haplotypes of Blastocystis sp. Identified in Faeces from Various Animal Groups in Algeria.Acta parasitologica · 2024Article
- Marked genetic diversity withinInternational journal for parasitology. Parasites and wildlife · 2024Article
- Molecular Prevalence and Subtypes Distribution ofTropical medicine and infectious disease · 2024Review
- Circulation and colonisation ofEpidemiology and infection · 2023Article
- Draft genomes of Blastocystis subtypes from human samples of Colombia.Parasites & vectors · 2023Article
- Identification and Molecular Characterization of Four NewMicroorganisms · 2022Article
- An update on the distribution ofHeliyon · 2022Article
- Diversity ofMicroorganisms · 2022Article
- Amplicon_sorter: A tool for reference-free amplicon sorting based on sequence similarity and for building consensus sequences.Ecology and evolution · 2022Article
- Molecular Detection and Characterization ofVeterinary sciences · 2021Article
- Wide Genetic Diversity ofMicroorganisms · 2021Article
- Next-generation sequencing reveals wide genetic diversity of Blastocystis subtypes in chickens including potentially zoonotic subtypes.Parasitology research · 2021Article
- Mind the Gap: New Full-Length Sequences ofMicroorganisms · 2021Article
- Identification of MultipleFrontiers in veterinary science · 2021Article
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3 authors.
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Abstract
backgroundBlastocystis sp. is one of the most common enteric parasites of humans and animals worldwide. It is well recognized that this ubiquitous protist displays a remarkable degree of genetic diversity in the SSU rRNA gene, which is currently the main gene used for defining Blastocystis subtypes. Yet, full-length reference sequences of this gene are available for only 16 subtypes of Blastocystis in part because of the technical difficulties associated with obtaining these sequences from complex samples.
methodsWe have developed a method using Oxford Nanopore MinION long-read sequencing and universal eukaryotic primers to produce full-length (> 1800 bp) SSU rRNA gene sequences for Blastocystis. Seven Blastocystis specimens representing five subtypes (ST1, ST4, ST10, ST11, and ST14) obtained both from cultures and feces were used for validation.
resultsWe demonstrate that this method can be used to produce highly accurate full-length sequences from both cultured and fecal DNA isolates. Full-length sequences were successfully obtained from all five subtypes including ST11 for which no full-length reference sequence currently exists and for an isolate that contained mixed ST10/ST14.
conclusionsThe suitability of the use of MinION long-read sequencing technology to successfully generate full-length Blastocystis SSU rRNA gene sequences was demonstrated. The ability to produce full-length SSU rRNA gene sequences is key in understanding the role of genetic diversity in important aspects of Blastocystis biology such as transmission, host specificity, and pathogenicity.
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