Evidence map›Paper›PMID 33239080›Full record

ArticleStem cell research & therapy2020

Human levator veli palatini muscle: a novel source of mesenchymal stromal cells for use in the rehabilitation of patients with congenital craniofacial malformations.

Daniela Franco Bueno, Gerson Shigueru Kabayashi, Carla Cristina Gomes Pinheiro, Daniela Y S Tanikawa, Cassio Eduardo Raposo-Amaral, Diogenes Laercio Rocha, José Ricardo Muniz Ferreira, Yoichiro Shibuya, Akishige Hokugo, Reza Jarrahy and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 6 institutions in 2 countries.

Daniela Franco BuenoHospital Sírio-Libanês, Instituto de Ensino e Pesquisa, São Paulo, SP, Brazil. dbuenousp@gmail.com.ORCID 0000-0001-5932-6134
Gerson Shigueru KabayashiUniversidade de São Paulo - USP, Instituto de Biociências, Centro de Pesquisa sobre o Genoma Humano e Células-Tronco, São Paulo, SP, Brazil.
Carla Cristina Gomes PinheiroHospital Sírio-Libanês, Instituto de Ensino e Pesquisa, São Paulo, SP, Brazil.
Daniela Y S TanikawaHospital Sírio-Libanês, Instituto de Ensino e Pesquisa, São Paulo, SP, Brazil.
Cassio Eduardo Raposo-AmaralHospital SOBRAPAR, Campinas, SP, Brazil.
Diogenes Laercio RochaHospital Sírio-Libanês, Instituto de Ensino e Pesquisa, São Paulo, SP, Brazil.
José Ricardo Muniz FerreiraInstituto Militar de Engenharia (IME), Departamento de Ciências de Materiais, Programa de Pós-graduação em Ciências de Materiais, Rio de Janeiro, RJ, Brazil.
Yoichiro ShibuyaDivision of Plastic and Reconstructive Surgery, David Geffen School of Medicine, University of California Los Angeles (UCLA), Los Angeles, CA, USA.
Akishige HokugoDivision of Plastic and Reconstructive Surgery, David Geffen School of Medicine, University of California Los Angeles (UCLA), Los Angeles, CA, USA.
Reza JarrahyDivision of Plastic and Reconstructive Surgery, David Geffen School of Medicine, University of California Los Angeles (UCLA), Los Angeles, CA, USA.
Patricia A ZuKDivision of Plastic and Reconstructive Surgery, David Geffen School of Medicine, University of California Los Angeles (UCLA), Los Angeles, CA, USA.
Maria Rita Passos-BuenoUniversidade de São Paulo - USP, Instituto de Biociências, Centro de Pesquisa sobre o Genoma Humano e Células-Tronco, São Paulo, SP, Brazil.
University of California, Los Angeles · USHospital Sírio-Libanês · BRInstituto Biológico · BRHospital do Servidor Público Municipal · BRHospital of Sobrapar · BRMilitary Institute of Engineering · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBone reconstruction in congenital craniofacial differences, which affect about 2-3% of newborns, has long been the focus of intensive research in the field of bone tissue engineering. The possibility of using mesenchymal stromal cells in regenerative medicine protocols has opened a new field of investigation aimed at finding optimal sources of multipotent cells that can be isolated via non-invasive procedures. In this study, we analyzed whether levator veli palatini muscle fragments, which can be readily obtained in non-invasive manner during palatoplasty in cleft palate patients, represent a novel source of MSCs with osteogenic potential.

methodsWe obtained levator veli palatini muscle fragments (3-5 mm

resultsFlow cytometry analysis showed that the isolated stromal cells were positive for mesenchymal stem cell antigens (CD29, CD44, CD73, CD90, and CD105) and negative for hematopoietic (CD34 and CD45) or endothelial cell markers (CD31). The cells successfully underwent osteogenic, chondrogenic, and adipogenic cell differentiation under appropriate cell culture conditions. Calvarial defects treated with CellCeram™ scaffolds seeded with the isolated levator veli palatini muscle cells showed greater bone healing compared to defects treated with acellular scaffolds.

conclusionCells derived from levator veli palatini muscle have phenotypic characteristics similar to other mesenchymal stromal cells, both in vitro and in vivo. Our findings suggest that these cells may have clinical relevance in the surgical rehabilitation of patients with cleft palate and other craniofacial anomalies characterized by significant bone deficit.

Indexed as

Cleft PalateMesenchymal Stem CellsPalatal MusclesAnimalsHumansInfant, NewbornMuscle, SkeletalOsteogenesisRatsBone reconstructionCraniofacial malformationsLevator veli palatini muscleMesenchymal stromal cellsOsteogenic differentiationScaffold

Identifiers

PMID33239080
PMCPMC7687766
OpenAlexW3109124591

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.