ArticleParasites & vectors2020
Surveillance of dengue virus in individual Aedes aegypti mosquitoes collected concurrently with suspected human cases in Tarlac City, Philippines.
Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 41 citations in OpenAlex.
- Detection of dengue virus serotypes in Aedes larvae: a multi-location study from Bhubaneswar, India, suggestive of vertical transmission.Virology journal · 2026Article
- Detection of Dengue virus serotype 2 (DENV-2) by RT-qPCR in Aedes aegypti in the state of Paraná, Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- The Frontier of Entomo-Virology: Applications and Tools for Virus and Vector Surveillance.Pathogens (Basel, Switzerland) · 2025Review
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- The Main Arboviruses and Virus Detection Methods in Vectors: Current Approaches and Future Perspectives.Pathogens (Basel, Switzerland) · 2025Review
- Study on the temporal and spatial distribution of Culex mosquitoes in Hanoi, Vietnam.Scientific reports · 2024Article
- Detecting the impacts of humidity, rainfall, temperature, and season on chikungunya, dengue and Zika viruses in Aedes albopictus mosquitoes from selected sites in Cebu city, Philippines.Virology journal · 2024Article
- Advancing pathogen genomics in resource-limited settings.Cell genomics · 2023Review
- Utility of CDC DENV1-4 real time PCR assay and trioplex assay for the diagnosis of dengue in patients with acute febrile illness.Virusdisease · 2023Article
- Comparison of Fan-Traps and Gravitraps forFrontiers in public health · 2022Article
- Entomological Investigation and Detection of Dengue Virus Type 1 inFrontiers in cellular and infection microbiology · 2022Article
- Towards Integrated Management of Dengue in Mumbai.Viruses · 2021Review
- Concurrent dengue infections: Epidemiology & clinical implications.The Indian journal of medical research · 2021Review
- The influence of roads on the fine-scale population genetic structure of the dengue vector Aedes aegypti (Linnaeus).PLoS neglected tropical diseases · 2021Article
- Dengue virus in humans and mosquitoes and their molecular characteristics in northeastern Thailand 2016-2018.PloS one · 2021Article
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8 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundVector control measures are critical for the prevention and reduction of dengue virus (DENV) transmission. Effective vector control is reliant not only on knowledge of mosquito abundance, but also on the timely and accurate detection of mosquito-borne infection. Mosquito-based virus surveillance programs typically rely on pool-based mosquito testing, although whether individual-based mosquito testing is a feasible alternative to this has not been widely studied. Applying an individual-based mosquito testing approach, we conducted a 1-month surveillance study of DENV in adult Aedes aegypti mosquitoes in homes of suspected dengue patients during the 2015 peak dengue season in Tarlac City, Philippines to more accurately assess the mosquito infection rate and identify the DENV serotypes and genotypes concurrently co-circulating in mosquitoes and patients there.
methodsWe performed a one-step multiplex real-time reverse transcription-polymerase chain reaction (RT-PCR) assay for the simultaneous detection and serotyping of DENV in patients and individual female Ae. aegypti mosquitoes. Additionally, we performed sequencing and phylogenetic analyses to further characterize the detected DENV serotypes in mosquitoes and patients at the genotype level.
resultsWe collected a total of 583 adult Ae. aegypti mosquitoes, of which we individually tested 359 female mosquitoes for the presence of DENV. Ten (2.8%) of the 359 female mosquitoes were positive for the presence of DENV. We detected DENV-1, DENV-2, and DENV-4 in the field-collected mosquitoes, which was consistent with the serotypes concurrently found in infected patients. Sequencing and phylogenetic analyses of the detected DENV serotypes based on the partial sequence of the evelope (E) gene revealed three genotypes concurrently present in the sampled mosquitoes and patients during the study period, namely DENV-1 genotype IV, DENV-2 Cosmopolitan genotype, and DENV-4 genotype II.
conclusionsWe demonstrated the utility of a one-step multiplex real-time RT-PCR assay for the individual-based DENV surveillance of mosquitoes. Our findings reinforce the importance of detecting and monitoring virus activity in local mosquito populations, which are critical for dengue prevention and control.
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