Evidence map›Paper›PMID 33238883›Full record

ArticleBMC neuroscience2020

Sex-dimorphic neuroestradiol regulation of ventromedial hypothalamic nucleus glucoregulatory transmitter and glycogen metabolism enzyme protein expression in the rat.

Md Main Uddin, Mostafa M H Ibrahim, Karen P Briski

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC neuroscience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.0field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Md Main UddinWillis-Knighton Endowed Professor of Pharmacy and Director, School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, University of Louisiana Monroe, 356 Bienville Building, 1800 Bienville Drive, Monroe, LA, 71201, USA.
Mostafa M H IbrahimWillis-Knighton Endowed Professor of Pharmacy and Director, School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, University of Louisiana Monroe, 356 Bienville Building, 1800 Bienville Drive, Monroe, LA, 71201, USA.
Karen P BriskiWillis-Knighton Endowed Professor of Pharmacy and Director, School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, University of Louisiana Monroe, 356 Bienville Building, 1800 Bienville Drive, Monroe, LA, 71201, USA. briski@ulm.edu.ORCID 0000-0002-7648-1395
University of Louisiana at Monroe · US

Funding

Estradiol Regulation of Hypothalamic Astrocyte GlycogenR01DK109382 · NIDDK · UNIVERSITY OF LOUISIANA AT MONROE · PI BRISKI, KAREN P · 2016 to 2020
$1.7M
NIDDK NIH HHS R01 DK109382
6 · The paper itself

Abstract

backgroundVentromedial hypothalamic nucleus (VMN) gluco-regulatory transmission is subject to sex-specific control by estradiol. The VMN is characterized by high levels of aromatase expression.

methodsThe aromatase inhibitor letrozole (LZ) was used with high-resolution microdissection/Western blot techniques to address the hypothesis that neuroestradiol exerts sex-dimorphic control of VMN neuronal nitric oxide synthase (nNOS) and glutamate decarboxylase

resultsVMN aromatase protein content was similar between sexes. Intracerebroventricular LZ infusion of testes-intact male and ovariectomized, estradiol-replaced female rats blocked insulin-induced hypoglycemic (IIH) up-regulation of this profile. LZ exerted sex-contingent effects on basal VMN nNOS and GAD expression, but blocked IIH-induced NO stimulation and GAD suppression in each sex. Sex-contingent LZ effects on basal and hypoglycemic patterns of GPbb and GPmm expression occurred at distinctive levels of the VMN. LZ correspondingly down- or up-regulated baseline pyruvate recycling pathway marker protein expression in males (glutaminase) and females (malic enzyme-1), and altered INS effects on those proteins.

conclusionsResults infer that neuroestradiol is required in each sex for optimal VMN metabolic transmitter signaling of hypoglycemic energy deficiency. Sex differences in VMN GP variant protein levels and sensitivity to aromatase may correlate with sex-dimorphic glycogen mobilization during this metabolic stress. Neuroestradiol may also exert sex-specific effects on glucogenic amino acid energy yield by actions on distinctive enzyme targets in each sex.

Indexed as

Sex CharacteristicsAnimalsAromatase InhibitorsEstradiolEstrogen Replacement TherapyFemaleGene Expression RegulationGlucoseGlutamate DecarboxylaseGlutaminaseGlycogenGlycogen SynthaseLetrozoleMalate DehydrogenaseMaleNitric Oxide SynthaseAromatase InhibitorsEstradiolGlucoseGlutamate DecarboxylaseGlutaminaseGlycogenGlycogen SynthaseLetrozoleMalate Dehydrogenasemalate dehydrogenase (decarboxylating)Nitric Oxide SynthaseAromataseGlutamate decarboxylase65/67Insulin-induced hypoglycemiaLetrozoleNeuronal nitric oxide synthaseVentromedial hypothalamic nucleus

Identifiers

PMID33238883
PMCPMC7687823
OpenAlexW3109104263

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.