ArticleNature2020
Progenitor identification and SARS-CoV-2 infection in human distal lung organoids.
Open access · bronzeAbstract read
In one paragraph
Article in Nature, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 270 papers, 2 of them syntheses that pooled it.
0numbers the graph read from it
0cells of the map it votes in
270citing papers in PubMed, 2 pooled it
35.3field-weighted citation impact, top 1% of its field
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
270 citing papers in PubMed, 2 syntheses or guidelines pooled it, 410 citations in OpenAlex.
- Advancing lung organoids toward clinical applications: a global perspective on research focus and future directions.Frontiers in medicine · 2025Pooled it
- Lung Organoids: Systematic Review of Recent Advancements and its Future Perspectives.Tissue engineering and regenerative medicine · 2024Pooled it
- Airway organoids reveal patterns of influenza A tropism and adaptation in wildlife species.Emerging microbes & infections · 2026Article
- A CRISPR activation screen identifies CH25H as a restriction factor against influenza viruses by targeting accessible cholesterol.Emerging microbes & infections · 2026Article
- Endowing Flexibility of Alveolar Type II Cells to Recapitulate Development From Lung Bud to Alveoli.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Delayed antiviral immune response in alveolar type 2 cells increases susceptibility to influenza virus in alveoli.Chinese medical journal · 2026Article
- Human airway organoids for bacterial-host interaction studies: methods, insights and translational promise.Thorax · 2026Review
- Early cell-autonomous and niche-mediated epithelial response to influenza infection in primary alveolar organoids.JCI insight · 2026Article
- Immune organoids for Africa: a roadmap for bridging the innovation gap in infectious diseases and cancer research.Journal of the Egyptian National Cancer Institute · 2026Review
- A comprehensive long RNA landscape of multi-regional porcine lung-derived small extracellular vesicles.BMC genomics · 2026Article
- Activation of ERBB4 Pathway Inhibits Pathological Transdifferentiation of Lung Epithelial Progenitors into CD66cAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Defective alveolar regeneration in emphysema: identifying dysfunctional cellular crosstalk and novel therapeutic opportunities for tissue repair.European respiratory review : an official journal of the European Respiratory Society · 2026Review
- Inducing physiological polarity and performing gene editing using CRISPR-Cas9 in human trophoblast organoids.Nature protocols · 2026Review
- Apical-out airway organoids as a pathophysiological model to study macrophage-mediated epithelial responses to microplastics.Materials today. Bio · 2026Article
- Organoids: generation strategies, applications, and future challenges.Stem cell research & therapy · 2026Review
- Advances in our understanding of distal progenitors in idiopathic pulmonary fibrosis: implications for novel therapeutics.The European respiratory journal · 2026Review
- Unlocking Alveolar Regeneration: AT2 Stem Cells, Signaling Networks, and Therapeutic Frontiers.Stem cell reviews and reports · 2026Review
- Translating Pharmacology from Animal Models to Organoids: Advancing Predictive Human Biology in Drug Discovery.Stem cell reviews and reports · 2026Review
- Interferon-γ selectively promotes survival of alveolar progenitor cells in a human lung organoid model.The EMBO journal · 2026Article
- A new tunable 3D alveolospheres model from human alveolar epithelial type 2 cells (AEC2) with reduced heterogeneity for studying cigarette smoke extract exposure.Respiratory research · 2026Article
210 more citing papers are in PubMed but not listed here.
Corrections and comments
- Update of
Authors and funding
34 authors at 6 institutions in 1 country.
Ameen A Salahudeen *Division of Hematology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.ORCID http://orcid.org/0000-0001-9037-3481
Shannon S Choi *Division of Hematology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.
Arjun RustagiDivision of Infectious Disease and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-6921-1012
Junjie ZhuStanford University School of Engineering, Department of Electrical Engineering, Stanford, CA, USA.ORCID http://orcid.org/0000-0001-7177-8694
Vincent van UnenDepartment of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.ORCID http://orcid.org/0000-0001-9339-8430
Sean M de la ODivision of Hematology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.
Ryan A FlynnStanford ChEM-H, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0001-5013-0442
Mar Margalef-CatalàDepartment of Pediatrics, Stanford University School of Medicine, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-6259-4348
António J M SantosDivision of Hematology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.
Jihang JuDivision of Hematology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.
Arpit BatishDivision of Hematology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.
Tatsuya UsuiDivision of Hematology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.
Grace X Y Zheng10x Genomics, Pleasanton, CA, USA.
Caitlin E EdwardsDepartment of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.ORCID http://orcid.org/0000-0003-4228-2192
Lisa E WagarDepartment of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.
Vincent LucaDepartment of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA.
Benedict AnchangDivision of Biomedical Data Science, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.
Monica NagendranDivision of Pulmonary, Allergy and Critical Care, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.
Khanh NguyenDivision of Gastroenterology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.
Daniel J HartDivision of Hematology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.
Jessica M Terry10x Genomics, Pleasanton, CA, USA.
Phillip Belgrader10x Genomics, Pleasanton, CA, USA.
Solongo B Ziraldo10x Genomics, Pleasanton, CA, USA.
Tarjei S Mikkelsen10x Genomics, Pleasanton, CA, USA.
Pehr B HarburyDepartment of Biochemistry, Stanford University School of Medicine, Stanford, CA, USA.
Jeffrey S GlennDepartment of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.
K Christopher GarciaDepartment of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA.ORCID http://orcid.org/0000-0001-9273-0278
Mark M DavisDepartment of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.ORCID http://orcid.org/0000-0001-6868-657X
Ralph S BaricDepartment of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.ORCID http://orcid.org/0000-0001-6827-8701
Chiara SabattiDivision of Biomedical Data Science, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.
Manuel R AmievaDepartment of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.
Catherine A BlishDivision of Infectious Disease and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA. cblish@stanford.edu.ORCID http://orcid.org/0000-0001-6946-7627
Tushar J DesaiDivision of Pulmonary, Allergy and Critical Care, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA. tdesai@stanford.edu.ORCID http://orcid.org/0000-0002-8794-5319
Calvin J KuoDivision of Hematology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA. cjkuo@stanford.edu.ORCID http://orcid.org/0000-0002-7427-5985
Stanford University · USHoward Hughes Medical Institute · USStanford Medicine · USUniversity of North Carolina at Chapel Hill · USChan Zuckerberg Initiative (United States) · USIllinois College · US
Funding
Using a tonsil organoid system to probe conditions for the induction of protective antibody and T cell responses to influenza.U19AI057229 · NIAID · STANFORD UNIVERSITY · PI Mark Morris Davis · 2003 to 2026
$88.5MMEDICAL SCIENTIST TRAINING PROGRAMT32GM007365 · NIGMS · STANFORD UNIVERSITY · PI CHUA, KATRIN F · 1985 to 2021
$33.8MStanford/UNC Biomimetic U19 Research CenterU19AI116484 · NIAID · STANFORD UNIVERSITY · PI GREENBERG, HARRY BERNARD · 2015 to 2025
$13.5MRegulation of actively proliferating and quiescent intestinal stem cellsU01DK085527 · NIDDK · STANFORD UNIVERSITY · PI KUO, CALVIN J · 2009 to 2023
$6.9MEMERGING INFECTIOUS DISEASEST32AI007502 · NIAID · STANFORD UNIVERSITY · PI Prasanna Jagannathan, DAVID A. RELMAN · 1995 to 2026
$6.8MIntestinal Stem Cell Consortium Coordinating CenterU01DK085532 · NIDDK · BECKMAN RESEARCH INSTITUTE/CITY OF HOPE · PI NILAND, JOYCE CAROL · 2009 to 2018
$6.5MHuman antibody-based countermeasures against the Wuhan Coronavirus SARS-CoV-2R01AI157155 · NIAID · WASHINGTON UNIVERSITY · PI BARIC, RALPH S, CROWE, JAMES E · 2020 to 2024
$6.0MOrganoid-based Discovery of Oncogenic Drivers and Treatment Resistance MechanismsU01CA217851 · NCI · STANFORD UNIVERSITY · PI CURTIS, CHRISTINA N, JI, HANLEE P · 2017 to 2021
$4.7MIdentification of Cooperative Genetic Alterations in the Pathogenesis of Oral CancerU01DE025188 · NIDCR · STANFORD UNIVERSITY · PI GEVAERT, OLIVIER, KUO, CALVIN J · 2015 to 2018
$3.8MFunctional Analysis of Oncogenic Networks in Primary OrganoidsU01CA176299 · NCI · STANFORD UNIVERSITY · PI JI, HANLEE P, KUO, CALVIN J · 2013 to 2016
$3.6MSingle-cell Analysis of Normal and Perturbed Dynamic Biological SystemsZIAES103350 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI ANCHANG, BENEDICT · 2020 to 2025
$3.5MLimited Competition for the Continuation of the Intestinal Stem Cell Consortium Coordinating CenterU24DK085532 · NIDDK · BECKMAN RESEARCH INSTITUTE/CITY OF HOPE · PI NILAND, JOYCE CAROL · 2019 to 2023
$2.3MHoward Hughes Medical InstituteNCI NIH HHS U01 CA176299NCI NIH HHS U01 CA217851NCI NIH HHS UH3 CA255135NHLBI NIH HHS R01 HL142549NHLBI NIH HHS UG3 HL145623NIAID NIH HHS R01 AI157155NIAID NIH HHS R56 AI111460NIAID NIH HHS T32 AI007502NIAID NIH HHS U19 AI057229NIAID NIH HHS U19 AI116484NIDCR NIH HHS K08 DE027730NIDCR NIH HHS U01 DE025188NIDDK NIH HHS U01 DK085527NIDDK NIH HHS U01 DK085532NIDDK NIH HHS U24 DK085532NIGMS NIH HHS T32 GM007365NIH HHS 5R01HL14254902NIH HHS DK11572802NIH HHS T32 AI007502-23NIH HHS T32 GM007365-44Wellcome Trust
Abstract
The distal lung contains terminal bronchioles and alveoli that facilitate gas exchange. Three-dimensional in vitro human distal lung culture systems would strongly facilitate the investigation of pathologies such as interstitial lung disease, cancer and coronavirus disease 2019 (COVID-19) pneumonia caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here we describe the development of a long-term feeder-free, chemically defined culture system for distal lung progenitors as organoids derived from single adult human alveolar epithelial type II (AT2) or KRT5
Indexed as
Models, BiologicalTissue Culture TechniquesAlveolar Epithelial CellsCell DifferentiationCell DivisionClone CellsCOVID-19HumansInfluenza A Virus, H1N1 SubtypeIntegrin alpha6Integrin beta4In Vitro TechniquesKeratin-5LungOrganoidsPneumonia, ViralIntegrin alpha6Integrin beta4ITGA6 protein, humanITGB4 protein, humanKeratin-5KRT5 protein, humanTNFRSF12A protein, humanTWEAK Receptor
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