Evidence map›Paper›PMID 33238158›Full record

ArticleCell chemical biology2021

Chemically Induced Cell Wall Stapling in Bacteria.

Sylvia L Rivera, Akbar Espaillat, Arjun K Aditham, Peyton Shieh, Chris Muriel-Mundo, Justin Kim, Felipe Cava, M Sloan Siegrist

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell chemical biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. A synthetic 5,3-cross-link in the cell wall of rod-shaped Gram-positive bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 2 countries.

Sylvia L RiveraDepartment of Microbiology, University of Massachusetts, Amherst, MA 01003, USA.
Akbar EspaillatLaboratory for Molecular Infection Medicine, Department of Molecular Biology, Umeå University, Umeå 90187, Sweden.
Arjun K AdithamDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Stanford ChEM-H (Chemistry, Engineering, and Medicine for Human Health), Stanford University, Stanford, CA 94305, USA.
Peyton ShiehDepartment of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Chris Muriel-MundoDepartment of Microbiology, University of Massachusetts, Amherst, MA 01003, USA.
Justin KimDepartment of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02215, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
Felipe CavaLaboratory for Molecular Infection Medicine, Department of Molecular Biology, Umeå University, Umeå 90187, Sweden. Electronic address: felipe.cava@umu.se.
M Sloan SiegristDepartment of Microbiology, University of Massachusetts, Amherst, MA 01003, USA; Molecular and Cellular Biology Program, University of Massachusetts, Amherst, MA 01003, USA. Electronic address: siegrist@umass.edu.
University of Massachusetts Amherst · USUmeå University · SEHarvard University · USMassachusetts Institute of Technology · USStanford University · US

Funding

UMass Amherst PREP ProgramR25GM086264 · NIGMS · UNIVERSITY OF MASSACHUSETTS AMHERST · PI PEYTON, SHELLY R., RICHARDSON, HEATHER NOËL · 2009 to 2023
$4.7M
Stanford Chem-H Chemistry/Biology Interface Predoctoral Training ProgramT32GM120007 · NIGMS · STANFORD UNIVERSITY · PI BERTOZZI, CAROLYN · 2016 to 2020
$1.2M
NIGMS NIH HHS R25 GM086264NIGMS NIH HHS T32 GM120007
6 · The paper itself

Abstract

Transpeptidation reinforces the structure of cell-wall peptidoglycan, an extracellular heteropolymer that protects bacteria from osmotic lysis. The clinical success of transpeptidase-inhibiting β-lactam antibiotics illustrates the essentiality of these cross-linkages for cell-wall integrity, but the presence of multiple, seemingly redundant transpeptidases in many species makes it challenging to determine cross-link function. Here, we present a technique to link peptide strands by chemical rather than enzymatic reaction. We employ biocompatible click chemistry to induce triazole formation between azido- and alkynyl-d-alanine residues that are metabolically installed in the peptidoglycan of Gram-positive or Gram-negative bacteria. Synthetic triazole cross-links can be visualized using azidocoumarin-d-alanine, an amino acid derivative that undergoes fluorescent enhancement upon reaction with terminal alkynes. Cell-wall stapling protects Escherichia coli from treatment with the broad-spectrum β-lactams ampicillin and carbenicillin. Chemical control of cell-wall structure in live bacteria can provide functional insights that are orthogonal to those obtained by genetics.

Indexed as

Anti-Bacterial AgentsBacteriaBacterial Infectionsbeta-LactamsCell WallCross-Linking ReagentsEscherichia coliHumansPeptidesAnti-Bacterial Agentsbeta-LactamsCross-Linking ReagentsPeptidesantibioticbacteriacell wallclick chemistrycross-linkingd-amino acidmetabolic labelingpeptidoglycantranspeptidase

Identifiers

PMID33238158
PMCPMC7897265
OpenAlexW3107016198

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.