ArticleReproductive sciences (Thousand Oaks, Calif.)2021
Effects of HIF-1α on Spermatogenesis of Varicocele Rats by Regulating VEGF/PI3K/Akt Signaling Pathway.
Article in Reproductive sciences (Thousand Oaks, Calif.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 27 citations in OpenAlex.
- Observation or treatment of paediatric varicoceles: revisiting the evidence.Nature reviews. Urology · 2026Review
- Impact of exercise and vitamin D on cardiac tissue morphology and pathological changes in rats: role of endostatin regulation in VEGF and lipid profile improvement.European journal of applied physiology · 2026Article
- Exercise Training Ameliorates The Tramadol-Induced Toxicity on VEGF/VEGFRs/PI3K/AKT Signaling Pathway in Testicular Tissue after Tramadol Withdrawal.International journal of fertility & sterility · 2026Article
- Venocentric perspective on varicocele: summarizing mechanisms and explorations.Frontiers in physiology · 2026Review
- Deciphering the Therapeutic Mechanisms of Wuzi Yanzong Pill for Asthenozoospermia: A Synergistic Approach Combining Bioinformatics and Molecular Dynamics.Cell biochemistry and biophysics · 2025Article
- Contribution and Regulation of HIF-1α in Testicular Injury Induced by Diabetes Mellitus.Biomolecules · 2025Review
- Pathophysiological effects of hypoxia on testis function and spermatogenesis.Nature reviews. Urology · 2025Review
- The microRNA-mediated apoptotic signaling axis in male reproduction: a possible and targetable culprit in male infertility.Cell biology and toxicology · 2025Review
- Insights into the Molecular Mechanisms of Bushen Huoxue Decoction in Breast CancerCurrent computer-aided drug design · 2025Article
- Article
- Experimental Varicocele Impairs DNA Methylation and Demethylation in Germ Cells, TESE, and Epididymal Spermatozoa, Impacting Active DNA Demethylation in Zygotes.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- Exploring the mechanisms of Gui Zhi Fu Ling Wan on varicocele via network pharmacology and molecular docking.Andrologia · 2022Article
- Application of CRISPR/Cas Technology in Spermatogenesis Research and Male Infertility Treatment.Genes · 2022Review
- Long noncoding RNAs regulated spermatogenesis in varicocele-induced spermatogenic dysfunction.Cell proliferation · 2022Article
- Role of Oxidative Stress in Varicocele.Frontiers in genetics · 2022Review
- Integrative Analysis of the lncRNA-Associated ceRNA Regulatory Network Response to Hypoxia in Alveolar Type II Epithelial Cells of Tibetan Pigs.Frontiers in veterinary science · 2022Article
- Hypoxia, oxidative stress, and immune evasion: a trinity of the trichothecenes T-2 toxin and deoxynivalenol (DON).Archives of toxicology · 2021Review
- The Abscopal Effects of Cranial Irradiation Induce Testicular Damage in Mice.Frontiers in physiology · 2021Article
- Effects of Environmental and Pathological Hypoxia on Male Fertility.Frontiers in cell and developmental biology · 2021Review
- Critical Roles of VEGFR1, VEGFR2, VEGFR3, BAX, and BCL-2 in the Pathogenesis of Varicose Veins: Unveiling Molecular Mechanisms.American journal of men's healthArticle
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hypoxia-inducible factor-1α (HIF-1α) participates in the regulation of spermatogenic function in rats with varicocele (VC), and the PI3K/Akt pathway plays an important role in it. In the present research, we applied the CRISPR/Cas9 gene editing technique to silence the HIF-1α gene of VC rat testis, to explore the effect of HIF-1α on apoptosis of spermatogenic cells in VC rats through the PI3K/Akt pathway. Sprague Dawley rats were randomly assigned to four groups, including the normal rat group (group N), VC model group (group V), VC + HIF-1α-lentivirus group (group H), and VC + luciferase-lentivirus group (group L). Apoptosis of spermatogenic cells in rat testis was tested by TUNEL Kit. The morphologic changes of seminiferous tubules were viewed by a light microscope. Expressions of VEGF, Akt, p-Akt, p70S6K, and p-p70S6K were detected by means of Western blot, immunofluorescence, or immunohistochemistry methods. One-way ANOVA was applied to analyze the diverseness between groups. Compared with group N, the distribution of germ cells was disordered, apoptosis of spermatogenic cells increased significantly, and the expression of VEGF, p-Akt, and p-p70S6K was also increased in group V. Compared with group V, the damage of seminiferous epithelium in group H was improved, and the arrangement of the seminiferous epithelium was almost orderly. Apoptosis of spermatogenic cells decreased significantly, and the expression of VEGF, p-Akt, and p-p70S6K protein was decreased (P < 0.05). There was no significant difference between group N and group H (P > 0.05).In conclusion, HIF-1α is regulated by hypoxia in rats with varicocele to regulate its downstream gene VEGF which regulates spermatogenesis, and the PI3K/Akt signaling pathway plays a regulatory role in this process.
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