Evidence map›Paper›PMID 33237489›Full record

ArticleJournal, genetic engineering & biotechnology2020

In silico modelling and characterization of eight blast resistance proteins in resistant and susceptible rice cultivars.

R Chandrakanth, L Sunil, L Sadashivaiah, N S Devaki

Open access · diamondAbstract read
In one paragraph

Article in Journal, genetic engineering & biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

R ChandrakanthDepartment of Molecular Biology, Yuvaraja's College, University of Mysore, Mysuru, Karnataka, 570005, India.
L SunilDepartment of Plant Cell Biotechnology, CSIR-Central Food Technological Research Institute, Mysuru, 570020, India.
L SadashivaiahDepartment of Molecular Biology, Yuvaraja's College, University of Mysore, Mysuru, Karnataka, 570005, India.
N S DevakiDepartment of Molecular Biology, Yuvaraja's College, University of Mysore, Mysuru, Karnataka, 570005, India. devakineerkaje@gmail.com.ORCID http://orcid.org/0000-0002-7694-4230
University of Mysore · INCentral Food Technological Research Institute · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundNucleotide-binding site-leucine-rich repeat (NBS-LRR) resistance genes are the largest class of plant resistance genes which play an important role in the plant defense response. These genes are better conserved than others and function as a recognition-based immune system in plants through their encoded proteins.

resultsHere, we report the effect of Magnaporthe oryzae, the rice blast pathogen inoculation in resistant BR2655 and susceptible HR12 rice cultivars. Transcriptomic profiling was carried out to analyze differential gene expression in these two cultivars. A total of eight NBS-LRR uncharacterized resistance proteins (RP1, RP2, RP3, RP4, RP5, RP6, RP7, and RP8) were selected in these two cultivars for in silico modeling. Modeller 9.22 and SWISS-MODEL servers were used for the homology modeling of eight RPs. ProFunc server was utilized for the prediction of secondary structure and function. The CDvist Web server and Interpro scan server detected the motif and domains in eight RPs. Ramachandran plot of eight RPs confirmed that the modeled structures occupied favorable positions.

conclusionsFrom the present study, computational analysis of these eight RPs may afford insights into their role, function, and valuable resource for studying the intricate details of the plant defense mechanism. Furthermore, the identification of resistance proteins is useful for the development of molecular markers linked to resistance genes.

Indexed as

Homology modelingIn silico analysisNBS-LRRResistance proteinsRice blast

Identifiers

PMID33237489
PMCPMC7688789
OpenAlexW3107189053

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.