ArticleeLife2020
The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motif.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- The preprophase band is dispensable for robust cell division orientation in wood-forming cambium stem cells.The New phytologist · 2026Article
- Dexamethasone-inducible LhGR/pOp system: simple and flexible spatiotemporal control of gene expression.Planta · 2026Review
- The developing leaf of the wild grass Brachypodium distachyon at single-cell resolution.The Plant cell · 2026Article
- Arabidopsis OST1 homologs of barley are involved in stomatal regulation.Journal of experimental botany · 2026Article
- Mechanisms controlling the plasma membrane targeting and the nanodomain organization of the plant SPFH protein HIR2.The Plant journal : for cell and molecular biology · 2026Article
- MUTE drives asymmetric divisions to form stomatal subsidiary cells in Crassulaceae succulents.Science advances · 2026Article
- Retrieval from vacuolar and endosomal compartments underpinning the neofunctionalization of SNARE in plants.Nature plants · 2025Article
- Detection of Cytosolic Ion Concentrations by the Trans-Golgi Network/Early Endosome is Important for Salt Tolerance.bioRxiv : the preprint server for biology · 2025Article
- ATG8ylation-mediated tonoplast invagination mitigates vacuole damage.Nature communications · 2025Article
- Resolving the two-body problem: A postulated role for the V0 sector of the V0V1-ATPase in exosome biogenesis and multivesicular body fate.Molecular biology of the cell · 2025Article
- Dual role of BdMUTE during stomatal development in the model grass Brachypodium distachyon.Development (Cambridge, England) · 2024Article
- Regulation of V-ATPase by Jasmonic Acid: Possible Role of Persulfidation.International journal of molecular sciences · 2023Article
- Structural and Functional Diversity of Two ATP-Driven Plant Proton Pumps.International journal of molecular sciences · 2023Review
- The cytosolic N-terminal domain of V-ATPase a-subunits is a regulatory hub targeted by multiple signals.Frontiers in molecular biosciences · 2023Review
- Efficient Agrobacterium-mediated transformation and genome editing ofFrontiers in plant science · 2023Article
- Article
- Tonoplast proton pumps regulate nuclear spacing of female gametophytesFrontiers in plant science · 2022Article
- The Plant V-ATPase.Frontiers in plant science · 2022Review
- Multiple functions of the vacuole in plant growth and fruit quality.Molecular horticulture · 2021Review
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
The V-ATPase is a versatile proton-pump found in a range of endomembrane compartments yet the mechanisms governing its differential targeting remain to be determined. In Arabidopsis, VHA-a1 targets the V-ATPase to the TGN/EE whereas VHA-a2 and VHA-a3 are localized to the tonoplast. We report here that the VHA-a1 targeting domain serves as both an ER-exit and as a TGN/EE-retention motif and is conserved among seed plants. In contrast, Marchantia encodes a single VHA-isoform that localizes to the TGN/EE and the tonoplast in Arabidopsis. Analysis of CRISPR/Cas9 generated null alleles revealed that VHA-a1 has an essential function for male gametophyte development but acts redundantly with the tonoplast isoforms during vegetative growth. We propose that in the absence of VHA-a1, VHA-a3 is partially re-routed to the TGN/EE. Our findings contribute to understanding the evolutionary origin of V-ATPase targeting and provide a striking example that differential localization does not preclude functional redundancy.
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