Evidence map›Paper›PMID 33236954›Full record

ArticleOrganogenesis2020

Differentiation of Human Parthenogenetic Embryonic Stem Cells into Functional Hepatocyte-like Cells.

Rui Liang, Zhiqiang Wang, Xiangyang Kong, Xiaoxiao Xiao, Tianxing Chen, Hui Yang, Ying Li, Xingqi Zhao

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Organogenesis, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 2 countries.

Rui LiangDepartment of Pathology, The Second Hospital of Tianjin Medical University , Tianjin, China.
Zhiqiang WangDepartment of General Surgery, The Second Hospital of Tianjin Medical University , Tianjin, China.
Xiangyang KongSchool of Medicine, Kunming University of Science and Technology , Kunming, China.
Xiaoxiao XiaoFaculty of Chinese medicine, Macau University of Science and Technology , Macao, China.ORCID 0000-0001-6562-9430
Tianxing ChenDepartment of Pathology, The First People's Hospital of Yunnan Province , Kunming, China.
Hui YangDepartment of Pathology, The First People's Hospital of Yunnan Province , Kunming, China.
Ying LiDepartment of Pathology, The First People's Hospital of Yunnan Province , Kunming, China.
Xingqi ZhaoCollege of Life Sciences, Nanjing Normal University , Nanjing, China.
First People's Hospital of Yunnan Province · CNSecond Hospital of Tianjin Medical University · CNKunming University of Science and Technology · CNMacau University of Science and Technology · MONanjing Normal University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Stem cell and tissue engineering-based therapies for acute liver failure (ALF) have been limited by the lack of an optimal cell source. We aimed to determine the suitability of human parthenogenetic embryonic stem cells (hPESCs) for the development of strategies to treat ALF. We studied the ability of human parthenogenetic embryonic stem cells (hPESCs) with high whole-genome SNP homozygosity, which were obtained by natural activation during in vitro fertilization (IVF), to differentiate into functional hepatocyte-like cells in vitro by monolayer plane orientation. hPESCs were induced on a single-layer flat plate for 21 d in complete medium with the inducers activin A, FGF-4, BMP-2, HGF, OSM, DEX, and B27. Polygonal cell morphology and binuclear cells were observed after 21 d of induction by using an inverted microscope. RT-qPCR results showed that the levels of hepatocyte-specific genes such as AFP, ALB, HNF4a, CYP3A4, SLCO1B3, and ABCC2 significantly increased after induction. Immunocytochemical assay showed CK18 and Hepa expression in the induced cells. Indocyanine green (ICG) staining showed that the cells had the ability to absorb and metabolize dyes. Detection of marker proteins and urea in cell culture supernatants showed that the cells obtained after 21 d of induction had synthetic and secretory functions. The typical ultrastructure of liver cells was observed using TEM after 21 d of induction. The results indicate that naturally activated hPESCs can be induced to differentiate into hepatocellular cells by monolayer planar induction.

Indexed as

Cell DifferentiationBiomarkersCell- and Tissue-Based TherapyCells, CulturedEmbryonic Stem CellsGene Expression Regulation, DevelopmentalHepatocytesHep G2 CellsHumansMultidrug Resistance-Associated Protein 2ParthenogenesisABCC2 protein, humanBiomarkersMultidrug Resistance-Associated Protein 2acute liver failurehepatic differentiationhomozygosityHuman parthenogenetic embryonic stem cells

Identifiers

PMID33236954
PMCPMC7714486
OpenAlexW3109533904

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.