ArticleJournal of integrative plant biology2021
Roles of DEMETER in regulating DNA methylation in vegetative tissues and pathogen resistance.
Article in Journal of integrative plant biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- The dynamic landscape of plant DNA demethylation: mechanisms, functions, and environmental responses.Plant cell reports · 2026Review
- Occupancy-based mechanism is the chief mode of ROS1 function in preventing DNA hypermethylation.Nature plants · 2026Article
- Versatile molecular tools enabling customizable DNA methylation editing in Arabidopsis.Nature communications · 2025Article
- Article
- Molecular mechanisms and biological functions of active DNA demethylation in plants.Epigenetics & chromatin · 2025Review
- The DNA methylation-demethylation balance prevents development of multiple megaspore mother cells in Arabidopsis.The Plant cell · 2025Article
- Review
- Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.Current issues in molecular biology · 2024Review
- Genome-Wide Analysis of DNA Demethylases in Land Plants and Their Expression Pattern in Rice.Plants (Basel, Switzerland) · 2024Article
- Epigenetic weapons of plants against fungal pathogens.BMC plant biology · 2024Review
- Mapping of adult plant recessive resistance to anthracnose in Indian common bean landrace Baspa/KRC 8.Molecular biology reports · 2024Article
- Base Excision DNA Repair in Plants:International journal of molecular sciences · 2023Review
- Elm tree defences against a specialist herbivore are moderately primed by an infestation in the previous season.Tree physiology · 2023Article
- DDM1-mediated gene body DNA methylation is associated with inducible activation of defense-related genes in Arabidopsis.Genome biology · 2023Article
- A Conserved, Serine-Rich Protein Plays Opposite Roles in N-Mediated Immunity against TMV and N-Triggered Cell Death.Viruses · 2022Article
- Dysfunction of histone demethylase IBM1 in Arabidopsis causes autoimmunity and reshapes the root microbiome.The ISME journal · 2022Article
- Transcriptional regulation of plant innate immunity.Essays in biochemistry · 2022Review
- Plant DNA Methylation: An Epigenetic Mark in Development, Environmental Interactions, and Evolution.International journal of molecular sciences · 2022Review
- DNA methylation-free Arabidopsis reveals crucial roles of DNA methylation in regulating gene expression and development.Nature communications · 2022Article
- The Ups and Downs of PlantFrontiers in plant science · 2022Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
DNA methylation is an epigenetic mark important for genome stability and gene expression. In Arabidopsis thaliana, the 5-methylcytosine DNA glycosylase/demethylase DEMETER (DME) controls active DNA demethylation during the reproductive stage; however, the lethality of loss-of-function dme mutations has made it difficult to assess DME function in vegetative tissues. Here, we edited DME using clustered regularly interspaced short palindromic repeats (CRISPR) /CRISPR-associated protein 9 and created three weak dme mutants that produced a few viable seeds. We also performed central cell-specific complementation in a strong dme mutant and combined this line with mutations in the other three Arabidopsis demethylase genes to generate the dme ros1 dml2 dml3 (drdd) quadruple mutant. A DNA methylome analysis showed that DME is required for DNA demethylation at hundreds of genomic regions in vegetative tissues. A transcriptome analysis of the drdd mutant revealed that DME and the other three demethylases are important for plant responses to biotic and abiotic stresses in vegetative tissues. Despite the limited role of DME in regulating DNA methylation in vegetative tissues, the dme mutants showed increased susceptibility to bacterial and fungal pathogens. Our study highlights the important functions of DME in vegetative tissues and provides valuable genetic tools for future investigations of DNA demethylation in plants.
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