ArticlePlant biotechnology journal2021
Modification of starch composition, structure and properties through editing of TaSBEIIa in both winter and spring wheat varieties by CRISPR/Cas9.
Article in Plant biotechnology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers, 1 of them a synthesis that pooled it.
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Who cites it
62 citing papers in PubMed, 1 synthesis or guideline pooled it, 157 citations in OpenAlex.
- Exploring and exploiting genetics and genomics for sweetpotato improvement: Status and perspectives.Plant communications · 2022Pooled it
- Crop biofortification for global food security: advances in genetic engineering and biotechnological approaches.Plant molecular biology · 2026Review
- Genome-Wide Identification of Ovarian Tumor Proteases Gene Family and Knockout ofPlants (Basel, Switzerland) · 2026Article
- Moderate Regulation of SBEIIb Gene Expression by Editing Cis-Regulatory Elements Enhances the Resistant Starch Content in Rice.Plant biotechnology journal · 2026Article
- Modification of starch synthesis in food crops using CRISPR/Cas9 gene editing technology for changing climate.Stress biology · 2026Review
- Starch characteristics distinguish malt from non-malt barley and can be modulated viaFrontiers in plant science · 2026Article
- Integrated Multi-Omics Reveals the Molecular Basis Underlying Wheat Grain Development and IdentifiesPlants (Basel, Switzerland) · 2025Article
- From convention to innovation: the role of genetic modification and genome editing in Australian wheat breeding.AoB PLANTS · 2025Review
- Turbo-charging crop improvement: harnessing multiplex editing for polygenic trait engineering and beyond.The Plant journal : for cell and molecular biology · 2025Review
- The Role ofInternational journal of molecular sciences · 2025Review
- Genome editing in maize and sorghum: A comprehensive review of CRISPR/Cas9 and emerging technologies.The plant genome · 2025Review
- Engineering a robust Cas12i3 variant-mediated wheat genome editing system.Plant biotechnology journal · 2025Article
- Enhancing tiny millets through genome editing: current status and future prospects.Molecular genetics and genomics : MGG · 2025Review
- CRISPR activation: identifying and using novel genes for plant disease resistance breeding.Frontiers in genome editing · 2025Review
- CRISPR/Cas9: a sustainable technology to enhance climate resilience in major Staple Crops.Frontiers in genome editing · 2025Review
- Advances in genome editing in plants within an evolving regulatory landscape, with a focus on its application in wheat breeding.Journal of plant biochemistry and biotechnology · 2025Review
- Review
- Drought-tolerant wheat for enhancing global food security.Functional & integrative genomics · 2024Review
- The Potential of CRISPR/Cas9 to Circumvent the Risk Factor NeurotoxinCurrent issues in molecular biology · 2024Review
- Advancements of CRISPR-Mediated Base Editing in Crops and Potential Applications inInternational journal of molecular sciences · 2024Review
2 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Foods high in amylose content and resistant starch (RS) offer great potential to improve human health and lower the risk of serious noninfectious diseases. Common wheat (Triticum aestivum L.) is a major staple food crop globally. However, the RS contents in the grains of modern wheat varieties are low. Here, we report the generation of high-amylose wheat through targeted mutagenesis of TaSBEIIa in a modern winter wheat cv Zhengmai 7698 (ZM) and a spring wheat cv Bobwhite by CRISPR/Cas9, respectively. We generated a series of transgene-free mutant lines either with partial or triple-null TasbeIIa alleles in ZM and Bobwhite, respectively. Analyses of starch composition, structure and properties revealed that the effects of partial or triple-null alleles were dosage dependent with triple-null lines demonstrated more profound impacts on starch composition, fine structures of amylopectin and physiochemical and nutritional properties. The flours of triple-null lines possessed significantly increased amylose, RS, protein and soluble pentosan contents which benefit human health. Baking quality analyses indicated that the high-amylose flours may be used as additives or for making cookies. Collectively, we successfully modified the starch composition, structure and properties through targeted mutagenesis of TaSBEIIa by CRISPR/Cas9 in both winter and spring wheat varieties and generated transgene-free high-amylose wheat. Our finding provides deep insights on the role of TaSBEIIa in determining starch composition, structure, properties and end-use quality in different genetic backgrounds and improving RS content with multiple breeding and end-use applications in cereal crop species through genome editing for health benefits.
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