Evidence map›Paper›PMID 33235312›Full record

ArticleScientific reports2020

Multiple gene substitution by Target-AID base-editing technology in tomato.

Johan Hunziker, Keiji Nishida, Akihiko Kondo, Sanae Kishimoto, Tohru Ariizumi, Hiroshi Ezura

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 54 citations in OpenAlex.

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  4. From Lab to Field: CRISPRing Major CultivatedInternational journal of molecular sciences · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Johan HunzikerGraduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan.
Keiji NishidaGraduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan.
Akihiko KondoGraduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan.
Sanae KishimotoInstitute of Vegetable and Floricultural Science, NARO, Tsukuba, Ibaraki, Japan.
Tohru AriizumiFaculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan.
Hiroshi EzuraFaculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan. ezura.hiroshi.fa@u.tsukuba.ac.jp.
University of Tsukuba · JPKobe University · JPInstitute of Vegetable and Floriculture Science · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The use of Target activation-induced cytidine deaminase (Target-AID) base-editing technology with the CRISPR-Cas 9 system fused with activation-induced cytidine deaminase (AID) resulted in the substitution of a cytidine with a thymine. In previous experiments focusing on a single target gene, this system has been reported to work in several plant species, including tomato (Solanum lycopersicum L.). In this research, we used Target-AID technology to target multiple genes related to carotenoid accumulation in tomato. We selected 3 genes, SlDDB1, SlDET1 and SlCYC-B, for their roles in carotenoid accumulation. Among 12 edited T

Indexed as

AllelesBase PairingCarotenoidsCRISPR-Cas SystemsCytidine DeaminaseGene EditingPlant ProteinsSolanum lycopersicumCarotenoidsCytidine DeaminasePlant Proteins

Identifiers

PMID33235312
PMCPMC7686336
OpenAlexW3107839316

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.