ArticleScientific reports2020
Macroscopic fluorescence-lifetime imaging of NADH and protoporphyrin IX improves the detection and grading of 5-aminolevulinic acid-stained brain tumors.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 40 citations in OpenAlex.
- Fluorescence lifetime imaging of 5-ALA-induced PpIX and autofluorescence for detecting infiltrating glioblastoma margins in patients.Biomedical optics express · 2026Article
- Optical express-biopsy of gliomas using macroscopic fluorescence lifetime imaging.Biomedical optics express · 2025Article
- Review
- Towards Optical Biopsy in Glioma Surgery.International journal of molecular sciences · 2025Review
- Widefield frequency-domain autofluorescence lifetime imaging for detecting breast cancer in murine xenograft tumor tissues.Journal of biomedical optics · 2025Article
- Fluorescence-Guided Surgery Using 5-Aminolevulinic Acid/Protoporphyrin IX in Brain Metastases.Neurosurgery practice · 2024Review
- Review
- Altered metabolism and DAM-signatures in female brains and microglia with aging.Brain research · 2024Article
- Miniature, multi-dichroic instrument for measuring the concentration of multiple fluorophores.Biomedical optics express · 2024Article
- Coupling a recurrent neural network to SPAD TCSPC systems for real-time fluorescence lifetime imaging.Scientific reports · 2024Article
- 5-ALA induced PpIX fluorescence spectroscopy in neurosurgery: a review.Frontiers in neuroscience · 2024Review
- Altered Metabolism and DAM-signatures in Female Brains and Microglia with Aging.bioRxiv : the preprint server for biology · 2023Article
- Label-free fluorescence microscopy: revisiting the opportunities with autofluorescent molecules and harmonic generations as biosensors and biomarkers for quantitative biology.Biophysical reviews · 2023Review
- In vivo characterization of the human glioblastoma infiltrative edge with label-free intraoperative fluorescence lifetime imaging.Biomedical optics express · 2023Article
- Review
- Computational approaches in rheumatic diseases - Deciphering complex spatio-temporal cell interactions.Computational and structural biotechnology journal · 2023Review
- Intraoperative microscopic autofluorescence detection and characterization in brain tumors using stimulated Raman histology and two-photon fluorescence.Frontiers in oncology · 2023Article
- Novel intraoperative strategies for enhancing tumor control: Future directions.Neuro-oncology · 2022Review
- First in patient assessment of brain tumor infiltrative margins using simultaneous time-resolved measurements of 5-ALA-induced PpIX fluorescence and tissue autofluorescence.Journal of biomedical optics · 2022Article
- Metabolic-imaging of human glioblastoma live tumors: A new precision-medicine approach to predict tumor treatment response early.Frontiers in oncology · 2022Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Maximal safe tumor resection remains the key prognostic factor for improved prognosis in brain tumor patients. Despite 5-aminolevulinic acid-based fluorescence guidance the neurosurgeon is, however, not able to visualize most low-grade gliomas (LGG) and infiltration zone of high-grade gliomas (HGG). To overcome the need for a more sensitive visualization, we investigated the potential of macroscopic, wide-field fluorescence lifetime imaging of nicotinamide adenine dinucleotide (NADH) and protoporphyrin IX (PPIX) in selected human brain tumors. For future intraoperative use, the imaging system offered a square field of view of 11 mm at 250 mm free working distance. We performed imaging of tumor tissue ex vivo, including LGG and HGG as well as brain metastases obtained from 21 patients undergoing fluorescence-guided surgery. Half of all samples showed visible fluorescence during surgery, which was associated with significant increase in PPIX fluorescence lifetime. While the PPIX lifetime was significantly different between specific tumor tissue types, the NADH lifetimes did not differ significantly among them. However, mainly necrotic areas exhibited significantly lower NADH lifetimes compared to compact tumor in HGG. Our pilot study indicates that combined fluorescence lifetime imaging of NADH/PPIX represents a sensitive tool to visualize brain tumor tissue not detectable with conventional 5-ALA fluorescence.
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