Evidence map›Paper›PMID 33235226›Full record

ArticleScientific reports2020

Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing.

Norbert Moldován, Gábor Torma, Gábor Gulyás, Ákos Hornyák, Zoltán Zádori, Victoria A Jefferson, Zsolt Csabai, Miklós Boldogkői, Dóra Tombácz, Florencia Meyer and 1 more

Abstract read
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Article
  6. Nanopore sequencing in veterinary medicine: from concepts to clinical applications.Frontiers in cellular and infection microbiology · 2025
    Review
  7. Article
  8. Article
  9. Article
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  11. Article
  12. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Norbert MoldovánDepartment of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., 6720, Szeged, Hungary.
Gábor TormaDepartment of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., 6720, Szeged, Hungary.
Gábor GulyásDepartment of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., 6720, Szeged, Hungary.
Ákos HornyákInstitute for Veterinary Medical Research, Centre for Agricultural Research, Hungária krt. 21, 1143, Budapest, Hungary.
Zoltán ZádoriInstitute for Veterinary Medical Research, Centre for Agricultural Research, Hungária krt. 21, 1143, Budapest, Hungary.
Victoria A JeffersonDepartment of Biochemistry and Molecular Biology, Entomology and Plant Pathology, Mississippi State University, 408 Dorman Hall, 32 Creelman St., Box 9655, Starkville, MS, 39762, USA.
Zsolt CsabaiDepartment of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., 6720, Szeged, Hungary.
Miklós BoldogkőiDepartment of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., 6720, Szeged, Hungary.
Dóra TombáczDepartment of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., 6720, Szeged, Hungary.
Florencia MeyerDepartment of Biochemistry and Molecular Biology, Entomology and Plant Pathology, Mississippi State University, 408 Dorman Hall, 32 Creelman St., Box 9655, Starkville, MS, 39762, USA.
Zsolt BoldogkőiDepartment of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., 6720, Szeged, Hungary. boldogkoi.zsolt@med.u-szeged.hu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Long-read sequencing (LRS) has become a standard approach for transcriptome analysis in recent years. Bovine alphaherpesvirus 1 (BoHV-1) is an important pathogen of cattle worldwide. This study reports the profiling of the dynamic lytic transcriptome of BoHV-1 using two long-read sequencing (LRS) techniques, the Oxford Nanopore Technologies MinION, and the LoopSeq synthetic LRS methods, using multiple library preparation protocols. In this work, we annotated viral mRNAs and non-coding transcripts, and a large number of transcript isoforms, including transcription start and end sites, as well as splice variants of BoHV-1. Our analysis demonstrated an extremely complex pattern of transcriptional overlaps.

Indexed as

Gene Expression ProfilingHigh-Throughput Nucleotide SequencingAlternative SplicingBase SequenceCell LineGene Expression Regulation, ViralGenome, ViralHerpesvirus 1, BovineIntronsKineticsMolecular Sequence AnnotationPeptidesPromoter Regions, GeneticProtein IsoformsRNA, MessengerTime FactorsPeptidesProtein IsoformsRNA, Messenger

Identifiers

PMID33235226
PMCPMC7686369

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.