Evidence map›Paper›PMID 33233721›Full record

ArticleInternational journal of molecular sciences2020

Identification of Recurrent Mutations in the microRNA-Binding Sites of B-Cell Lymphoma-Associated Genes in Follicular Lymphoma.

Erika Larrea, Marta Fernandez-Mercado, José Afonso Guerra-Assunção, Jun Wang, Ibai Goicoechea, Ayman Gaafar, Izaskun Ceberio, Carmen Lobo, Jessica Okosun, Anton J Enright and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 60% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Small Differences and Big Changes: The Many Variables of MicroRNA Expression and Function in the Brain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024
    Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 6 institutions in 3 countries.

Erika LarreaMolecular Oncology Group, Biodonostia Research Institute, 20014 San Sebastián, Spain.
Marta Fernandez-MercadoMolecular Oncology Group, Biodonostia Research Institute, 20014 San Sebastián, Spain.ORCID 0000-0003-3458-9231
José Afonso Guerra-AssunçãoGreat Ormond Street Institute of Child Health, University College London (UCL), London WC1N 1EH, UK.ORCID 0000-0001-6593-403X
Jun WangBarts Cancer Institute, Queen Mary University of London, London EC1M 6BE, UK.ORCID 0000-0003-2509-9599
Ibai GoicoecheaMolecular Oncology Group, Biodonostia Research Institute, 20014 San Sebastián, Spain.ORCID 0000-0002-5329-2225
Ayman GaafarDepartment of Pathology, Cruces Hospital, 48903 Bilbao, Spain.ORCID 0000-0003-0324-2251
Izaskun CeberioHematology Department, Hospital Universitario Donostia, 20014 San Sebastián, Spain.
Carmen LoboDepartment of Pathology, Hospital Universitario Donostia, 20014 San Sebastián, Spain.
Jessica OkosunBarts Cancer Institute, Queen Mary University of London, London EC1M 6BE, UK.ORCID 0000-0001-6021-5044
Anton J EnrightDepartment of Pathology, University of Cambridge, Cambridge CB2 1QP, UK.
Jude FitzgibbonBarts Cancer Institute, Queen Mary University of London, London EC1M 6BE, UK.
Charles H LawrieMolecular Oncology Group, Biodonostia Research Institute, 20014 San Sebastián, Spain.ORCID 0000-0002-8882-1131
Biogipuzkoa Health Research Institute · ESQueen Mary University of London · GBHospital de Cruces · ESIkerbasque · ESUniversity College London · GBUniversity of Cambridge · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Follicular lymphoma (FL) is a common indolent B-cell lymphoma that can transform into the more aggressive transformed FL (tFL). However, the molecular process driving this transformation is uncertain. In this work, we aimed to identify microRNA (miRNA)-binding sites recurrently mutated in follicular lymphoma patients, as well as in transformed FL patients. Using whole-genome sequencing data from FL tumors, we discovered 544 mutations located in bioinformatically predicted microRNA-binding sites. We then studied these specific regions using targeted sequencing in a cohort of 55 FL patients, found 16 recurrent mutations, and identified a further 69 variants. After filtering for QC, we identified 21 genes with mutated miRNA-binding sites that were also enriched for B-cell-associated genes by Gene Ontology. Over 40% of mutations identified in these genes were present exclusively in tFL patients. We validated the predicted miRNA-binding sites of five of the genes by luciferase assay and demonstrated that the identified mutations in

Indexed as

Binding SitesCell Line, TumorCohort StudiesEnhancer of Zeste Homolog 2 ProteinHumansLondonLymphoma, FollicularLymphoma, Large B-Cell, DiffuseMicroRNAsMutationProto-Oncogene Proteins c-bcl-2Retrospective StudiesSpainBCL2 protein, humanEnhancer of Zeste Homolog 2 ProteinEZH2 protein, humanMicroRNAsMIRN144 microRNA, humanProto-Oncogene Proteins c-bcl-2diffuse large B-cell lymphoma (DLBCL)follicular lymphoma (FL)microRNAmutation

Identifiers

PMID33233721
PMCPMC7699894
OpenAlexW3101892279

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.