ArticleCell2020
Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics.
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Article in Cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41citing papers in PubMed
8.4field-weighted citation impact, top 2% of its field
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Who cites it
41 citing papers in PubMed, 71 citations in OpenAlex.
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- Decoding Molecular Network Dynamics in Cells: Advances in Multiplexed Live Imaging of Fluorescent Biosensors.Biosensors · 2025Review
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- Next-Generation Genetically Encoded Fluorescent Biosensors Illuminate Cell Signaling and Metabolism.Annual review of biophysics · 2024Review
- Nociceptor-immune interactomes reveal insult-specific immune signatures of pain.Nature immunology · 2024Article
Corrections and comments
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Authors and funding
19 authors at 6 institutions in 2 countries.
Changyang LinghuDepartment of Media Arts and Sciences, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA; McGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA; Center for Neurobiological Engineering, MIT, Cambridge, MA 02139, USA; Department of Electrical Engineering and Computer Science, MIT, Cambridge, MA 02139, USA.
Shannon L JohnsonDepartment of Media Arts and Sciences, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA; McGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA; Center for Neurobiological Engineering, MIT, Cambridge, MA 02139, USA.
Pablo A ValdesDepartment of Media Arts and Sciences, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA; Department of Neurosurgery, Harvard Medical School, Brigham and Women's Hospital, Boston, MA 02115, USA.
Or A ShemeshDepartment of Media Arts and Sciences, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA; McGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA; Center for Neurobiological Engineering, MIT, Cambridge, MA 02139, USA; Department of Biological Engineering, MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA; Department of Neurobiology and Pittsburgh Institute for Neurodegenerative Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Won Min ParkTim Taylor Department of Chemical Engineering, Kansas State University, Manhattan, KS 66506, USA.
Demian ParkDepartment of Media Arts and Sciences, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA; McGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA; Center for Neurobiological Engineering, MIT, Cambridge, MA 02139, USA.
Kiryl D PiatkevichDepartment of Media Arts and Sciences, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA; McGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA; Center for Neurobiological Engineering, MIT, Cambridge, MA 02139, USA; Department of Biological Engineering, MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA; School of Life Sciences, Westlake University, Hangzhou, Zhejiang 310024, China.
Asmamaw T WassieDepartment of Media Arts and Sciences, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA; McGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA; Center for Neurobiological Engineering, MIT, Cambridge, MA 02139, USA; Department of Biological Engineering, MIT, Cambridge, MA 02139, USA.
Yixi LiuDepartment of Media Arts and Sciences, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA; McGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA; Department of Electrical Engineering and Computer Science, MIT, Cambridge, MA 02139, USA; Center for Neurobiological Engineering, MIT, Cambridge, MA 02139, USA.
Bobae AnDepartment of Media Arts and Sciences, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA; McGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA; Center for Neurobiological Engineering, MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA.
Stephanie A BarnesDepartment of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA; Picower Institute for Learning and Memory, MIT, Cambridge, MA 02139, USA.
Orhan T CelikerDepartment of Media Arts and Sciences, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA; McGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA; Center for Neurobiological Engineering, MIT, Cambridge, MA 02139, USA; Department of Electrical Engineering and Computer Science, MIT, Cambridge, MA 02139, USA.
Chun-Chen YaoDepartment of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA.
Chih-Chieh Jay YuDepartment of Media Arts and Sciences, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA; McGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA; Center for Neurobiological Engineering, MIT, Cambridge, MA 02139, USA; Department of Biological Engineering, MIT, Cambridge, MA 02139, USA.
Ru WangDepartment of Media Arts and Sciences, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA; McGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA; Center for Neurobiological Engineering, MIT, Cambridge, MA 02139, USA.
Katarzyna P AdamalaDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA.
Mark F BearDepartment of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA; Picower Institute for Learning and Memory, MIT, Cambridge, MA 02139, USA.
Amy E KeatingDepartment of Biological Engineering, MIT, Cambridge, MA 02139, USA; Department of Biology, MIT, Cambridge, MA 02139, USA; Koch Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Edward S BoydenDepartment of Media Arts and Sciences, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA; McGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA; Center for Neurobiological Engineering, MIT, Cambridge, MA 02139, USA; Department of Biological Engineering, MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA; Koch Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Cambridge, MA 02139, USA. Electronic address: edboyden@mit.edu.
McGovern Institute for Brain Research · USBrigham and Women's Hospital · USHoward Hughes Medical Institute · USIIT@MIT · USKansas State University · USUniversity of Minnesota · US
Funding
Novel Platforms for Systematic Optical Control of Complex Neural Circuits In VivoR01DA029639 · NIDA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2010 to 2023
$6.7MExpansion MicroscopyR01EB024261 · NIBIB · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2017 to 2025
$4.6MRedesigning General Anesthesia (Admin Supp)R01GM104948 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S., BROWN, EMERY N · 2012 to 2016
$4.4MMillisecond-Timescale Whole-Brain Neural Activity Mapping in Health and DiseaseDP1NS087724 · NINDS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2013 to 2017
$3.9MHigh-Performance Imaging Through Scattering Living TissueR01DA045549 · NIDA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2017 to 2020
$3.5MMultidimensional Optimization of Voltage Indicators for In Vivo Neural Activity ImagingR01MH122971 · NIMH · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI HAN, XUE · 2020 to 2024
$3.4MRNA Scaffolds for Cell Specific Multiplexed Neural ObservationR01MH114031 · NIMH · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2017 to 2020
$2.8MScalable Cell- and Circuit-Targeted ElectrophysiologyR01NS102727 · NINDS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S., FOREST, CRAIG · 2017 to 2020
$2.2MOpto-Crown: Transparent skulls with embedded optics for cortex-wide cellular resolution imaging in freely moving miceRF1NS113287 · NINDS · UNIVERSITY OF MINNESOTA · PI BOYDEN, EDWARD S., KODANDARAMAIAH, SUHASA B · 2019 to 2019
$2.0MVascular Interfaces for Brain Imaging and StimulationR24MH106075 · NIMH · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI DESIMONE, ROBERT · 2014 to 2016
$1.4MA next-generation spatial light modulator for mapping of neural networksR44EB021054 · NIBIB · BOULDER NONLINEAR SYSTEMS, INC. · PI HOY, CHRISTOPHER LUK · 2016 to 2017
$993kHoward Hughes Medical InstituteNIBIB NIH HHS R01 EB024261NIBIB NIH HHS R44 EB021054NIDA NIH HHS R01 DA029639NIDA NIH HHS R01 DA045549NIGMS NIH HHS R01 GM104948NIMH NIH HHS R01 MH114031NIMH NIH HHS R01 MH122971NIMH NIH HHS R24 MH106075NINDS NIH HHS DP1 NS087724NINDS NIH HHS R01 NS102727NINDS NIH HHS RF1 NS113287
Abstract
In order to analyze how a signal transduction network converts cellular inputs into cellular outputs, ideally one would measure the dynamics of many signals within the network simultaneously. We found that, by fusing a fluorescent reporter to a pair of self-assembling peptides, it could be stably clustered within cells at random points, distant enough to be resolved by a microscope but close enough to spatially sample the relevant biology. Because such clusters, which we call signaling reporter islands (SiRIs), can be modularly designed, they permit a set of fluorescent reporters to be efficiently adapted for simultaneous measurement of multiple nodes of a signal transduction network within single cells. We created SiRIs for indicators of second messengers and kinases and used them, in hippocampal neurons in culture and intact brain slices, to discover relationships between the speed of calcium signaling, and the amplitude of PKA signaling, upon receiving a cAMP-driving stimulus.
Indexed as
Genes, ReporterOptical ImagingSignal TransductionAnimalsCalciumCyclic AMPCyclic AMP-Dependent Protein KinasesFemaleFluorescent DyesGreen Fluorescent ProteinsHeLa CellsHippocampusHumansMiceNeuronsPeptidesCalciumCyclic AMPCyclic AMP-Dependent Protein KinasesFluorescent DyesGreen Fluorescent ProteinsPeptidesProteinscalcium imagingcAMPfluorescent reporterslive-cell imagingprotein kinaseprotein scaffoldsignaling pathwaysignaling reporter islandssignal transductionspatial multiplexing
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