Evidence map›Paper›PMID 33232598›Full record

ArticleACS synthetic biology2020

Design and Characterization of Rapid Optogenetic Circuits for Dynamic Control in Yeast Metabolic Engineering.

Evan M Zhao, Makoto A Lalwani, Robert J Lovelett, Sergio A García-Echauri, Shannon M Hoffman, Christopher L Gonzalez, Jared E Toettcher, Ioannis G Kevrekidis, José L Avalos

Open access · greenAbstract read
In one paragraph

Article in ACS synthetic biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 49 citations in OpenAlex.

  1. Engineered Optogenetic Circuits In Yeast with Self-Sustained Outputs.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  2. Article
  3. Article
  4. Article
  5. Structural Basis of a Novel Heme Binding Bacterial One-Component Switch.bioRxiv : the preprint server for biology · 2026
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Review
  13. Article
  14. Review
  15. Article
  16. Article
  17. A Toolkit for Precise, Multigene Control inACS synthetic biology · 2022
    Article
  18. A Single-Component Blue Light-Induced System Based on EL222 inInternational journal of molecular sciences · 2022
    Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Evan M ZhaoDepartment of Chemical and Biological Engineering, Hoyt Laboratory 101, Princeton University, William Street, Princeton, New Jersey 08544, United States.
Makoto A LalwaniDepartment of Chemical and Biological Engineering, Hoyt Laboratory 101, Princeton University, William Street, Princeton, New Jersey 08544, United States.
Robert J LovelettDepartment of Chemical and Biological Engineering, Hoyt Laboratory 101, Princeton University, William Street, Princeton, New Jersey 08544, United States.ORCID 0000-0003-1428-0931
Sergio A García-EchauriDepartment of Chemical and Biological Engineering, Hoyt Laboratory 101, Princeton University, William Street, Princeton, New Jersey 08544, United States.
Shannon M HoffmanDepartment of Chemical and Biological Engineering, Hoyt Laboratory 101, Princeton University, William Street, Princeton, New Jersey 08544, United States.
Christopher L GonzalezDepartment of Chemical and Biological Engineering, Hoyt Laboratory 101, Princeton University, William Street, Princeton, New Jersey 08544, United States.
Jared E ToettcherDepartment of Molecular Biology, 140 Lewis Thomas Laboratory, Princeton University, Washington Road, Princeton, New Jersey 08544, United States.ORCID 0000-0002-1546-4030
Ioannis G KevrekidisDepartment of Chemical and Biological Engineering, Hoyt Laboratory 101, Princeton University, William Street, Princeton, New Jersey 08544, United States.ORCID 0000-0003-2220-3522
José L AvalosDepartment of Chemical and Biological Engineering, Hoyt Laboratory 101, Princeton University, William Street, Princeton, New Jersey 08544, United States.ORCID 0000-0002-7209-4208
Princeton University · USJohns Hopkins University · US

Funding

Harnessing optogenetics to diagnose and therapeutically rewire cancer cell signalingDP2EB024247 · NIBIB · PRINCETON UNIVERSITY · PI TOETTCHER, JARED E · 2016 to 2016
$2.4M
NIBIB NIH HHS DP2 EB024247
6 · The paper itself

Abstract

The use of optogenetics in metabolic engineering for light-controlled microbial chemical production raises the prospect of utilizing control and optimization techniques routinely deployed in traditional chemical manufacturing. However, such mechanisms require well-characterized, customizable tools that respond fast enough to be used as real-time inputs during fermentations. Here, we present OptoINVRT7, a new rapid optogenetic inverter circuit to control gene expression in

Indexed as

ButanolsGalactokinaseGene Expression Regulation, FungalLactic AcidLightMetabolic EngineeringOptogeneticsPlasmidsPromoter Regions, GeneticSaccharomyces cerevisiaeButanolsGalactokinaseisobutyl alcoholLactic Acid

Identifiers

PMID33232598
PMCPMC10399620
OpenAlexW3109855541

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.