Evidence map›Paper›PMID 33232563›Full record

ArticleMolecular ecology resources2021

Discriminating lineages of Batrachochytrium dendrobatidis using quantitative PCR.

Pria N Ghosh, Ruhan Verster, Thomas R Sewell, Simon J O'Hanlon, Lola M Brookes, Adrien Rieux, Trenton W J Garner, Ché Weldon, Matthew C Fisher

Open access · hybridAbstract read
In one paragraph

Article in Molecular ecology resources, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.8field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Archival mitogenomes identify invasion by theProceedings. Biological sciences · 2024
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  7. Environment predictsEcology and evolution · 2024
    Article
  8. Whole-mitochondrial genomes ofEcology and evolution · 2023
    Article
  9. Review
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 4 countries.

Pria N GhoshMRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, Imperial College London, London, UK.ORCID https://orcid.org/0000-0002-9668-6662
Ruhan VersterUnit for Environmental Sciences and Management, North-West University, Potchefstroom, South Africa.ORCID https://orcid.org/0000-0003-2974-1155
Thomas R SewellMRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, Imperial College London, London, UK.ORCID https://orcid.org/0000-0002-7239-4817
Simon J O'HanlonMRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, Imperial College London, London, UK.ORCID https://orcid.org/0000-0002-6852-3911
Lola M BrookesMRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, Imperial College London, London, UK.ORCID https://orcid.org/0000-0001-5354-0244
Adrien RieuxCIRAD, UMR, PVBMT, St Pierre, La Réunion, France.ORCID https://orcid.org/0000-0002-7221-0010
Trenton W J GarnerUnit for Environmental Sciences and Management, North-West University, Potchefstroom, South Africa.ORCID https://orcid.org/0000-0002-0336-9706
Ché WeldonUnit for Environmental Sciences and Management, North-West University, Potchefstroom, South Africa.ORCID https://orcid.org/0000-0001-6716-0787
Matthew C FisherMRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, Imperial College London, London, UK.ORCID https://orcid.org/0000-0002-1862-6402
London Centre for Neglected Tropical Disease Research · GBNorth-West University · ZAZoological Society of London · GBCentre de Coopération Internationale en Recherche Agronomique pour le Développement · FR

Funding

Leverhulme Trust RPG-2014-273Medical Research Council MR/R015600/1Morris Animal FoundationNatural Environment Research Council NERC NE/E006841/1
6 · The paper itself

Abstract

The ability to detect and monitor infectious disease in a phylogenetically informative manner is critical for their management. Phylogenetically informative diagnostic tests enable patterns of pathogen introduction or changes in the distribution of genotypes to be measured, enabling research into the ecology of the pathogen. Batrachochytrium dendrobatidis (Bd), a causative agent of chytridiomycosis in amphibian populations, emerged worldwide in the 21st century and is composed of six lineages which are display varying levels of virulence in their hosts. Research into the distribution, ecology and pathogenicity of these lineages has been hampered by an inability to type lineage efficiently. Here, we describe a lineage-specific TaqMan qPCR assay that differentiates the two lineages of Bd most commonly associated with chytridiomycosis: BdGPL and BdCAPE. We demonstrate how this assay can be used for the surveillance of wild populations of amphibians in Southern Africa using skin swabs, tissue samples and cultured isolates.

Indexed as

Africa, SouthernAmphibiansAnimalsBatrachochytriumMycosesPolymerase Chain ReactionVirulenceBatrachochytrium dendrobatidischytriddiagnosticlineageqPCR

Identifiers

PMID33232563
PMCPMC8651002
OpenAlexW3109411788

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.