ArticleThe EMBO journal2021
Intrinsically disordered protein PID-2 modulates Z granules and is required for heritable piRNA-induced silencing in the Caenorhabditis elegans embryo.
Article in The EMBO journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 30 citations in OpenAlex.
- TurboID proximity labeling reveals protein interaction networks for germline Argonautes.bioRxiv : the preprint server for biology · 2026Article
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- The intrinsically disordered protein SPE-56 is required for acrosomal-like exocytosis and fertility in Caenorhabditis elegans.Scientific reports · 2026Article
- TurboID-based proximity labeling identifies novel germline proteins that maintain E granule integrity and small RNA homeostasis in C. elegans.Science China. Life sciences · 2025Article
- A genetic framework for RNAi inheritance in Caenorhabditis elegans.EMBO reports · 2025Article
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- Selecting genes for analysis using historically contingent progress: from RNA changes to protein-protein interactions.Nucleic acids research · 2025Article
- Synthetic guide sequence to generate CRISPR-Cas9 entry strains inmicroPublication biology · 2025Article
- Selecting genes for analysis using historically contingent progress: from RNA changes to protein-protein interactions.bioRxiv : the preprint server for biology · 2024Article
- Inheritance of Stress Responses via Small Non-Coding RNAs in Invertebrates and Mammals.Epigenomes · 2023Review
- Caenorhabditis elegans germ granules are present in distinct configurations and assemble in a hierarchical manner.Development (Cambridge, England) · 2023Article
- A Molecular Analysis of the Aminopeptidase P-Related Domain of PID-5 fromBiomolecules · 2023Article
- The Caenorhabditis elegans TDRD5/7-like protein, LOTR-1, interacts with the helicase ZNFX-1 to balance epigenetic signals in the germline.PLoS genetics · 2022Article
- Review
- Proline-specific aminopeptidase P prevents replication-associated genome instability.PLoS genetics · 2022Article
- Nuage condensates: accelerators or circuit breakers for sRNA silencing pathways?RNA (New York, N.Y.) · 2022Review
- Two isoforms of the essential C. elegans Argonaute CSR-1 differentially regulate sperm and oocyte fertility.Nucleic acids research · 2021Article
- ZSP-1 is a Z granule surface protein required for Z granule fluidity and germline immortality in Caenorhabditis elegans.The EMBO journal · 2021Article
- Intrinsically disordered protein PID-2 modulates Z granules and is required for heritable piRNA-induced silencing in the Caenorhabditis elegans embryo.The EMBO journal · 2021Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Caenorhabditis elegans, the piRNA (21U RNA) pathway is required to establish proper gene regulation and an immortal germline. To achieve this, PRG-1-bound 21U RNAs trigger silencing mechanisms mediated by RNA-dependent RNA polymerase (RdRP)-synthetized 22G RNAs. This silencing can become PRG-1-independent and heritable over many generations, a state termed RNA-induced epigenetic gene silencing (RNAe). How and when RNAe is established, and how it is maintained, is not known. We show that maternally provided 21U RNAs can be sufficient for triggering RNAe in embryos. Additionally, we identify PID-2, a protein containing intrinsically disordered regions (IDRs), as a factor required for establishing and maintaining RNAe. PID-2 interacts with two newly identified and partially redundant eTudor domain-containing proteins, PID-4 and PID-5. PID-5 has an additional domain related to the X-prolyl aminopeptidase APP-1, and binds APP-1, implicating potential N-terminal proteolysis in RNAe. All three proteins are required for germline immortality, localize to perinuclear foci, affect size and appearance of RNA inheritance-linked Z granules, and are required for balancing of 22G RNA populations. Overall, our study identifies three new proteins with crucial functions in C. elegans small RNA silencing.
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