ArticleNucleic acids research2020
An RNA pseudoknot is essential for standby-mediated translation of the tisB toxin mRNA in Escherichia coli.
Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- An RNA structural switch controlling bacterial toxin translation.Nucleic acids research · 2026Article
- Article
- ProQ-associated small RNAs control motility in Vibrio cholerae.Nucleic acids research · 2025Article
- Article
- Disentangling the pseudoknots of toxin translation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- An RNA pseudoknot mediates toxin translation and antitoxin inhibition.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- TisB protein is the single molecular determinant underlying multiple downstream effects of ofloxacin inScience advances · 2024Article
- Recent advances in RNA structurome.Science China. Life sciences · 2022Review
- Biology and evolution of bacterial toxin-antitoxin systems.Nature reviews. Microbiology · 2022Review
- Development of RNA G-quadruplex (rG4)-targeting L-RNA aptamers by rG4-SELEX.Nature protocols · 2022Review
- Incoherent dual regulation by a SAM-II riboswitch controlling translation at a distance.RNA biology · 2022Article
- Elevated Expression of Toxin TisB Protects Persister Cells against Ciprofloxacin but Enhances Susceptibility to Mitomycin C.Microorganisms · 2021Article
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3 authors.
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Abstract
In response to DNA damage, Escherichia coli cells activate the expression of the toxin gene tisB of the toxin-antitoxin system tisB-istR1. Of three isoforms, only the processed, highly structured +42 tisB mRNA is active. Translation requires a standby site, composed of two essential elements: a single-stranded region located 100 nucleotides upstream of the sequestered RBS, and a structure near the 5'-end of the active mRNA. Here, we propose that this 5'-structure is an RNA pseudoknot which is required for 30S and protein S1-alone binding to the mRNA. Point mutations that prevent formation of this pseudoknot inhibit formation of translation initiation complexes, impair S1 and 30S binding to the mRNA, and render the tisB mRNA non-toxic in vivo. A set of mutations created in either the left or right arm of stem 2 of the pseudoknot entailed loss of toxicity upon overexpression of the corresponding mRNA variants. Combining the matching right-left arm mutations entirely restored toxicity levels to that of the wild-type, active mRNA. Finally, since many pseudoknots have high affinity for S1, we predicted similar pseudoknots in non-homologous type I toxin-antitoxin systems that exhibit features similar to that of tisB-IstR1, suggesting a shared requirement for standby acting at great distances.
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