Evidence map›Paper›PMID 33230288›Full record

ArticleScientific reports2020

Embryonic periventricular endothelial cells demonstrate a unique pro-neurodevelopment and anti-inflammatory gene signature.

Franciele Cristina Kipper, Cleide Angolano, Ravi Vissapragada, Mauricio A Contreras, Justin Moore, Manoj Bhasin, Christiane Ferran, Ajith J Thomas

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Franciele Cristina KipperDivision of Neurosurgery, Beth Israel Deaconess Medical Center, Boston, MA, 02215, USA.
Cleide AngolanoDivision of Vascular and Endovascular Surgery, Department of Surgery, Beth Israel Deaconess Medical Center, Boston, MA, 02215, USA.
Ravi VissapragadaDivision of Neurosurgery, Beth Israel Deaconess Medical Center, Boston, MA, 02215, USA.
Mauricio A ContrerasDivision of Vascular Surgery, Department of Surgery, Beth Israel Deaconess Medical Center, Boston, MA, 02215, USA.
Justin MooreDivision of Neurosurgery, Beth Israel Deaconess Medical Center, Boston, MA, 02215, USA.
Manoj BhasinBIDMC Genomics, Proteomics, Bioinformatics and Systems Biology Center, Beth Israel Deaconess Medical Center, Boston, MA, 02215, USA.
Christiane FerranDivision of Vascular and Endovascular Surgery, Department of Surgery, Beth Israel Deaconess Medical Center, Boston, MA, 02215, USA.
Ajith J ThomasDivision of Neurosurgery, Beth Israel Deaconess Medical Center, Boston, MA, 02215, USA. athomas6@bidmc.harvard.edu.
Beth Israel Deaconess Medical Center · USHarvard University · US

Funding

HARVARD-LONGWOOD RESEARCH TRAINING IN VASCULAR SURGERYT32HL007734 · NHLBI · BETH ISRAEL DEACONESS MEDICAL CENTER · PI FERRAN, CHRISTIANE, LOGERFO, FRANK W · 1993 to 2023
$10.5M
Genetic Engineering of Vein Bypass Grafts in Vascular and Cardiovascular SurgeryR01HL086741 · NHLBI · BETH ISRAEL DEACONESS MEDICAL CENTER · PI BHASIN, MANOJ, FERRAN, CHRISTIANE · 2007 to 2022
$7.4M
Bioengineering of vein grafts to resist intimal hyperplasiaR21EB024308 · NIBIB · BETH ISRAEL DEACONESS MEDICAL CENTER · PI FERRAN, CHRISTIANE · 2018 to 2019
$481k
NHLBI NIH HHS R01 HL086741NHLBI NIH HHS T32 HL007734NIBIB NIH HHS R21 EB024308NIH HHS R21EB024308
6 · The paper itself

Abstract

Brain embryonic periventricular endothelial cells (PVEC) crosstalk with neural progenitor cells (NPC) promoting mutual proliferation, formation of tubular-like structures in the former and maintenance of stemness in the latter. To better characterize this interaction, we conducted a comparative transcriptome analysis of mouse PVEC vs. adult brain endothelial cells (ABEC) in mono-culture or NPC co-culture. We identified > 6000 differentially expressed genes (DEG), regardless of culture condition. PVEC exhibited a 30-fold greater response to NPC than ABEC (411 vs. 13 DEG). Gene Ontology (GO) analysis of DEG that were higher or lower in PVEC vs. ABEC identified "Nervous system development" and "Response to Stress" as the top significantly different biological process, respectively. Enrichment in canonical pathways included HIF1A, FGF/stemness, WNT signaling, interferon signaling and complement. Solute carriers (SLC) and ABC transporters represented an important subset of DEG, underscoring PVEC's implication in blood-brain barrier formation and maintenance of nutrient-rich/non-toxic environment. Our work characterizes the gene signature of PVEC and their important partnership with NPC, underpinning their unique role in maintaining a healthy neurovascular niche, and in supporting brain development. This information may pave the way for additional studies to explore their therapeutic potential in neuro-degenerative diseases, such as Alzheimer's and Parkinson's disease.

Indexed as

Gene Expression Regulation, DevelopmentalAgingAnimalsBrainCarrier ProteinsCell LineCoculture TechniquesComplement System ProteinsEmbryo, MammalianEndothelial CellsFemaleFibroblast Growth Factor 1Gene Expression ProfilingGene OntologyHypoxia-Inducible Factor 1, alpha SubunitInterferonsCarrier ProteinsComplement System ProteinsFibroblast Growth Factor 1Hif1a protein, mouseHypoxia-Inducible Factor 1, alpha SubunitInterferonsWnt Proteins

Identifiers

PMID33230288
PMCPMC7683543
OpenAlexW3108310922

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.