Evidence map›Paper›PMID 33228781›Full record

ArticleClinical epigenetics2020

DNA methylation signatures to predict the cervicovaginal microbiome status.

Nuno R Nené, James Barrett, Allison Jones, Iona Evans, Daniel Reisel, John F Timms, Tobias Paprotka, Andreas Leimbach, Dorella Franchi, Nicoletta Colombo and 4 more

Open access · goldAbstract readMulticenter Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical epigenetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Article
  5. Review
  6. Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 6 institutions in 7 countries.

Nuno R NenéDepartment of Women's Cancer, EGA Institute for Women's Health, University College London, London, UK.
James BarrettDepartment of Women's Cancer, EGA Institute for Women's Health, University College London, London, UK.
Allison JonesDepartment of Women's Cancer, EGA Institute for Women's Health, University College London, London, UK.
Iona EvansDepartment of Women's Cancer, EGA Institute for Women's Health, University College London, London, UK.
Daniel ReiselDepartment of Women's Cancer, EGA Institute for Women's Health, University College London, London, UK.
John F TimmsDepartment of Women's Cancer, EGA Institute for Women's Health, University College London, London, UK.
Tobias PaprotkaEurofins Genomics Europe Sequencing, Constance, Germany.
Andreas LeimbachEurofins Genomics Europe Sequencing, Constance, Germany.
Dorella FranchiEuropeo Di Oncologia, IRCCS, Milan, Italy.
Nicoletta ColomboEuropeo Di Oncologia, IRCCS, Milan, Italy.
Line BjørgeDepartment of Obstetrics and Gynecology, Haukeland University Hospital, Bergen, Norway.
Michal ZikanHospital Na Bulovce, Prague, Czech Republic.
David CibulaDepartment of Obstetrics and Gynecology, General University Hospital in Prague, First Faculty of Medicine, Charles University, Prague, Czech Republic.
Martin WidschwendterDepartment of Women's Cancer, EGA Institute for Women's Health, University College London, London, UK. m.widschwendter@ucl.ac.uk.ORCID 0000-0002-7778-8380
London Women's Clinic · GBCharles University · CZEurofins (Germany) · DEIstituti di Ricovero e Cura a Carattere Scientifico · ITUniversität Innsbruck · ATHaukeland University Hospital · NO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe composition of the microbiome plays an important role in human health and disease. Whether there is a direct association between the cervicovaginal microbiome and the host's epigenome is largely unexplored.

resultsHere we analyzed a total of 448 cervicovaginal smear samples and studied both the DNA methylome of the host and the microbiome using the Illumina EPIC array and next-generation sequencing, respectively. We found that those CpGs that are hypo-methylated in samples with non-lactobacilli (O-type) dominating communities are strongly associated with gastrointestinal differentiation and that a signature consisting of 819 CpGs was able to discriminate lactobacilli-dominating (L-type) from O-type samples with an area under the receiver operator characteristic curve (AUC) of 0.84 (95% CI = 0.77-0.90) in an independent validation set. The performance found in samples with more than 50% epithelial cells was further improved (AUC 0.87) and in women younger than 50 years of age was even higher (AUC 0.91). In a subset of 96 women, the buccal but not the blood cell DNA showed the same trend as the cervicovaginal samples in discriminating women with L- from O-type cervicovaginal communities.

conclusionsThese findings strongly support the view that the epithelial epigenome plays an essential role in hosting specific microbial communities.

Indexed as

AdultCervix UteriCpG IslandsDNA MethylationEpigenomeEpithelial CellsFemaleHigh-Throughput Nucleotide SequencingHumansLactobacillusMicrobiotaMiddle AgedPredictive Value of TestsVaginaCervicovaginal microbiomeDNA methylationEpigenome–microbiome interactionPenalized regression

Identifiers

PMID33228781
PMCPMC7686703
OpenAlexW3109942895

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.