Evidence map›Paper›PMID 33228532›Full record

ArticleBMC genomics2020

Transcriptomic profiling of adipose tissue inflammation, remodeling, and lipid metabolism in periparturient dairy cows (Bos taurus).

David Salcedo-Tacuma, Jair Parales-Giron, Crystal Prom, Miguel Chirivi, Juliana Laguna, Adam L Lock, G Andres Contreras

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
3.8field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 44 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

David Salcedo-TacumaDepartment of Physiology, College of Natural Sciences, Michigan State University, East Lansing, MI, 48824, USA.
Jair Parales-GironDepartment of Animal Science, College of Agriculture and Natural Resources, Michigan State University, East Lansing, MI, 48824, USA.
Crystal PromDepartment of Animal Science, College of Agriculture and Natural Resources, Michigan State University, East Lansing, MI, 48824, USA.
Miguel ChiriviDepartment of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI, 48824, USA.
Juliana LagunaDepartment of Animal Science, College of Agriculture and Natural Resources, Michigan State University, East Lansing, MI, 48824, USA.
Adam L LockDepartment of Animal Science, College of Agriculture and Natural Resources, Michigan State University, East Lansing, MI, 48824, USA.
G Andres ContrerasDepartment of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI, 48824, USA. contre28@msu.edu.ORCID http://orcid.org/0000-0003-4969-2178
Michigan State University · US

Funding

Michigan Alliance for Animal Agriculture AA18-003National Institute of Food and Agriculture 2019-67015-29443National Institute of Food and Agriculture Pre-Doctoral Fellowship from USDA NIFA (award 2017-07578)
6 · The paper itself

Abstract

backgroundPeriparturient cows release fatty acid reserves from adipose tissue (AT) through lipolysis in response to the negative energy balance induced by physiological changes related to parturition and the onset of lactation. However, lipolysis causes inflammation and structural remodeling in AT that in excess predisposes cows to disease. The objective of this study was to determine the effects of the periparturient period on the transcriptomic profile of AT using NGS RNAseq.

resultsSubcutaneous AT samples were collected from Holstein cows (n = 12) at 11 ± 3.6 d before calving date (PreP) and at 6 ± 1d (PP1) and 13 ± 1.4d (PP2) after parturition. Differential expression analyses showed 1946 and 1524 DEG at PP1 and PP2, respectively, compared to PreP. Functional Enrichment Analysis revealed functions grouped in categories such as lipid metabolism, molecular transport, energy production, inflammation, and free radical scavenging to be affected by parturition and the onset of lactation (FDR < 0.05). Inflammation related genes such as TLR4 and IL6 were categorized as upstream lipolysis triggers. In contrast, FASN, ELOVL6, ACLS1, and THRSP were identified as upstream inhibitors of lipid synthesis. Complement (C3), CXCL2, and HMOX1 were defined as links between inflammatory pathways and those involved in the generation of reactive oxygen species.

conclusionsResults offer a comprehensive characterization of gene expression dynamics in periparturient AT, identify upstream regulators of AT function, and demonstrate complex interactions between lipid mobilization, inflammation, extracellular matrix remodeling, and redox signaling in the adipose organ.

Indexed as

Lipid MetabolismTranscriptomeAdipose TissueAnimalsCattleDietEnergy MetabolismFemaleInflammationLactationParturitionPregnancyAdipose tissue inflammationLipogenesisLipolysisPeriparturient period

Identifiers

PMID33228532
PMCPMC7686742
OpenAlexW3110264245

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.