Evidence map›Paper›PMID 33228526›Full record

ArticleBMC plant biology2020

A full-length transcriptome and gene expression analysis reveal genes and molecular elements expressed during seed development in Gnetum luofuense.

Nan Deng, Chen Hou, Boxiang He, Fengfeng Ma, Qingan Song, Shengqing Shi, Caixia Liu, Yuxin Tian

Open access · goldAbstract read
In one paragraph

Article in BMC plant biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Nan DengHunan Academy of Forestry, Changsha, Hunan, No.658 Shaoshan Road, Tianxin District, Changsha, 410004, China.
Chen HouGuangdong Academy of Forestry, Guangzhou, 510520, China.
Boxiang HeGuangdong Academy of Forestry, Guangzhou, 510520, China.
Fengfeng MaHunan Academy of Forestry, Changsha, Hunan, No.658 Shaoshan Road, Tianxin District, Changsha, 410004, China.
Qingan SongHunan Academy of Forestry, Changsha, Hunan, No.658 Shaoshan Road, Tianxin District, Changsha, 410004, China.
Shengqing ShiState Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, No. 1 Dongxiaofu, Xiangshan Road, Haidian, Beijing, 100091, China.
Caixia LiuHunan Academy of Forestry, Changsha, Hunan, No.658 Shaoshan Road, Tianxin District, Changsha, 410004, China. lcaixia1989@163.com.
Yuxin TianHunan Academy of Forestry, Changsha, Hunan, No.658 Shaoshan Road, Tianxin District, Changsha, 410004, China. tianyuxineco@163.com.ORCID http://orcid.org/0000-0003-2066-9599
Guangdong Academy of Forestry · CNHunan Research Academy of Environmental Sciences · CNChinese Academy of Forestry · CN

Funding

Forestry Science and Technology Innovation Project of Hunan Province HNGYL-2019-01Natural Science Foundation of Guangdong Province No. 2018A0303130163Special Fund for Hunan Forestry Science and Technology XLK201812
6 · The paper itself

Abstract

backgroundGnetum is an economically important tropical and subtropical gymnosperm genus with various dietary, industrial and medicinal uses. Many carbohydrates, proteins and fibers accumulate during the ripening of Gnetum seeds. However, the molecular mechanisms related to this process remain unknown.

resultsWe therefore assembled a full-length transcriptome from immature and mature G. luofuense seeds using PacBio sequencing reads. We identified a total of 5726 novel genes, 9061 alternative splicing events, 3551 lncRNAs, 2160 transcription factors, and we found that 8512 genes possessed at least one poly(A) site. In addition, gene expression comparisons of six transcriptomes generated by Illumina sequencing showed that 14,323 genes were differentially expressed from an immature stage to a mature stage with 7891 genes upregulated and 6432 genes downregulated. The expression of 14 differentially expressed transcription factors from the MADS-box, Aux/IAA and bHLH families was validated by qRT-PCR, suggesting that they may have important roles in seed ripening of G. luofuense.

conclusionsThese findings provide a valuable molecular resource for understanding seed development of gymnosperms.

Indexed as

TranscriptomeAlternative SplicingCycadopsidaGnetumHigh-Throughput Nucleotide SequencingPlant ProteinsRNA, Long NoncodingRNA, PlantSeedsSequence Analysis, RNATranscription FactorsPlant ProteinsRNA, Long NoncodingRNA, PlantTranscription FactorsFull-length transcriptomeFunctional genesGnetaleslncRNASeed

Identifiers

PMID33228526
PMCPMC7685604
OpenAlexW3109198272

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.