Evidence map›Paper›PMID 33228016›Full record

ArticleToxics2020

An Embryonic Zebrafish Model to Screen Disruption of Gut-Vascular Barrier upon Exposure to Ambient Ultrafine Particles.

Kyung In Baek, Yi Qian, Chih-Chiang Chang, Ryan O'Donnell, Ehsan Soleimanian, Constantinos Sioutas, Rongsong Li, Tzung K Hsiai

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Refractive Index-Corrected Light-Sheet Microscopy for Macro-View Cardiovascular Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Kyung In BaekDepartment of Bioengineering and Medicine, University of California, Los Angeles, CA 90095, USA.ORCID 0000-0001-9388-2070
Yi QianDepartment of Bioengineering and Medicine, University of California, Los Angeles, CA 90095, USA.ORCID 0000-0001-8054-6887
Chih-Chiang ChangDepartment of Bioengineering and Medicine, University of California, Los Angeles, CA 90095, USA.
Ryan O'DonnellDepartment of Bioengineering and Medicine, University of California, Los Angeles, CA 90095, USA.
Ehsan SoleimanianDepartment of Civil and Environmental Engineering, University of Southern California, Los Angeles, CA 90089, USA.
Constantinos SioutasDepartment of Civil and Environmental Engineering, University of Southern California, Los Angeles, CA 90089, USA.ORCID 0000-0001-5146-0857
Rongsong LiCollege of Health Sciences and Environmental Engineering, Shenzhen Technology University, Shenzhen 518118, China.
Tzung K HsiaiDepartment of Bioengineering and Medicine, University of California, Los Angeles, CA 90095, USA.
University of California, Los Angeles · USUniversity of Southern California · USShenzhen Technology University · CN

Funding

NIH HHS R01HL083015, R01HL111437, R01HL129727, R01HL118650, I01 BX004356
6 · The paper itself

Abstract

Epidemiological studies have linked exposure to ambient particulate matter (PM) with gastrointestinal (GI) diseases. Ambient ultrafine particles (UFP) are the redox-active sub-fraction of PM2.5, harboring elemental and polycyclic aromatic hydrocarbons from urban environmental sources including diesel and gasoline exhausts. The gut-vascular barrier (GVB) regulates paracellular trafficking and systemic dissemination of ingested microbes and toxins. Here, we posit that acute UFP ingestion disrupts the integrity of the intestinal barrier by modulating intestinal Notch activation. Using zebrafish embryos, we performed micro-gavage with the fluorescein isothiocynate (FITC)-conjugated dextran (FD10, 10 kDa) to assess the disruption of GVB integrity upon UFP exposure. Following micro-gavage, FD10 retained in the embryonic GI system, migrated through the cloaca. Conversely, co-gavaging UFP increased transmigration of FD10 across the intestinal barrier, and FD10 fluorescence occurred in the venous capillary plexus. Ingestion of UFP further impaired the mid-intestine morphology. We performed micro-angiogram of FD10 to corroborate acute UFP-mediated disruption of GVB. Transient genetic and pharmacologic manipulations of global Notch activity suggested Notch regulation of the GVB. Overall, our integration of a genetically tractable embryonic zebrafish and micro-gavage technique provided epigenetic insights underlying ambient UFP ingestion disrupts the GVB.

Indexed as

gut-vascular barriermicro-gavageNotch signalingultrafine particleszebrafish

Identifiers

PMID33228016
PMCPMC7711522
OpenAlexW3104216581

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.