ReviewJournal of neuroscience methods2021
Can transsynaptic viral strategies be used to reveal functional aspects of neural circuitry?
Review in Journal of neuroscience methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 45 citations in OpenAlex.
- Selective genetic targeting of the mouse efferent vestibular nucleus identifies monosynaptic inputs and indicates function as multimodal integrator.Journal of neurophysiology · 2026Article
- Preparation and application of YFV-17D-derived Anterograde Trans-neuronal Viral Vectors for Neuroscience Research.bioRxiv : the preprint server for biology · 2026Article
- Transsynaptic viral tools in neural circuit analysis: from anatomical mapping to functional interrogation.Frontiers in neural circuits · 2026Review
- Sources of Synaptic Input to Neurons in the Nucleus Accumbens Shell That Project to the Ventral Pallidum.The Journal of comparative neurology · 2025Article
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- Characterization of direct Purkinje cell outputs to the brainstem.bioRxiv : the preprint server for biology · 2024Article
- Disrupted basolateral amygdala circuits supports negative valence bias in depressive states.Translational psychiatry · 2024Article
- Synaptic Connectivity and Electrophysiological Properties of the Nucleus of the Lateral Olfactory Tract.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Coordinated head direction representations in mouse anterodorsal thalamic nucleus and retrosplenial cortex.eLife · 2024Article
- Brain-wide circuit-specific targeting of astrocytes.Cell reports methods · 2023Article
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- Re-formation of synaptic connectivity in dissociated human stem cell-derived retinal organoid cultures.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
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- Ascertaining cells' synaptic connections and RNA expression simultaneously with barcoded rabies virus libraries.Nature communications · 2022Article
- The impact of phosphorylated PTEN at threonine 366 on cortical connectivity and behaviour.Brain : a journal of neurology · 2022Article
- All-viral tracing of monosynaptic inputs to single birthdate-defined neurons in the intact brain.Cell reports methods · 2022Article
- Article
- Dendritic Domain-Specific Sampling of Long-Range Axons Shapes Feedforward and Feedback Connectivity of L5 Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Viruses have proved instrumental to elucidating neuronal connectivity relationships in a variety of organisms. Recent advances in genetic technologies have facilitated analysis of neurons directly connected to a defined starter population. These advances have also made viral transneuronal mapping available to the broader neuroscience community, where one-step rabies virus mapping has become routine. This method is commonly used to identify inputs onto defined cell populations, to demonstrate the quantitative proportion of inputs coming from specific brain regions, or to compare input patterns between two or more cell populations. Furthermore, the number of inputs labeled is often assumed to reflect the number of synaptic connections, and these viruses are commonly believed to label strong synapses more efficiently than weak synapses. While these maps are often interpreted to provide a quantitative estimate of the synaptic landscape onto starter cell populations, in fact very little is known about how transneuronal transmission takes place. We do not know how these viruses transmit between neurons, if they display biases in the cell types labeled, or even if transmission is synapse-specific. In this review, we discuss the experimental evidence against or in support of key concepts in viral tracing, focusing mostly on the use of one-step rabies input mapping and related methods. Does spread of these viruses occur specifically through synaptic connections, preferentially through synapses, or non-specifically? How efficient is viral transneuronal transmission, and is this efficiency equal in all cell types? And lastly, to what extent does viral labeling reflect functional connectivity?
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.