ArticlePloS one2020
Improved yellow-green split fluorescent proteins for protein labeling and signal amplification.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Development of CRISPR/Cas13-based analytical tools to study RNA-protein interactions.Nucleic acids research · 2026Article
- Efficient Endogenous Tagging in the Sea Urchin,bioRxiv : the preprint server for biology · 2026Article
- Endogenous autophagosome reporters reveal redundant and non-redundant roles of LGG-1 and LGG-2 inbioRxiv : the preprint server for biology · 2026Article
- Intensity modulation of trichromatic split fluorescent proteins for live cell mapping.Cell reports methods · 2026Article
- Bipartite Genetically Encoded Biosensors to Sense Calcium Ion Dynamics at Membrane-Membrane Contact Sites.Analytical chemistry · 2025Article
- Accessible and accurate cytometry analysis of adherent cells using fluorescence microscopes.Scientific reports · 2025Article
- Efficient endogenous protein labelling in Dictyostelium using CRISPR/Cas9 knock-in and split fluorescent proteins.PloS one · 2025Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Tissue-specific and endogenous protein labeling with split fluorescent proteins.Developmental biology · 2024Article
- High-throughput optimized prime editing mediated endogenous protein tagging for pooled imaging of protein localization.bioRxiv : the preprint server for biology · 2024Article
- Probing and imaging phospholipid dynamics in live cells.Life metabolism · 2024Review
- GRK2 kinases in the primary cilium initiate SMOOTHENED-PKA signaling in the Hedgehog cascade.PLoS biology · 2024Article
- Expanding the Cell-Free Reporter Protein Toolbox by Employing a Split mNeonGreen System to Reduce Protein Synthesis Workload.ACS synthetic biology · 2024Article
- Article
- Tissue-specific and endogenous protein labeling with split fluorescent proteins.bioRxiv : the preprint server for biology · 2024Article
- CRISPR-Cas9-Mediated Knock-In Approach to Insert the GFPMethods in molecular biology (Clifton, N.J.) · 2023Article
- ATP13A1 prevents ERAD of folding-competent mislocalized and misoriented proteins.Molecular cell · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The flexibility and versatility of self-complementing split fluorescent proteins (FPs) have enabled a wide range of applications. In particular, the FP1-10/11 split system contains a small fragment that facilitates efficient generation of endogenous-tagged cell lines and animals as well as signal amplification using tandem FP11 tags. To improve the FP1-10/11 toolbox we previously developed, here we used a combination of directed evolution and rational design approaches, resulting in two mNeonGreen (mNG)-based split FPs (mNG3A1-10/11 and mNG3K1-10/11) and one mClover-based split FP (CloGFP1-10/11). mNG3A1-10/11 and mNG3K1-10/11 not only enhanced the complementation efficiency at low expression levels, but also allowed us to demonstrate signal amplification using tandem mNG211 fragments in mammalian cells.
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