ReviewPeriodontology 20002021
Optimizing the quality of clinical studies on oral microbiome: A practical guide for planning, performing, and reporting.
Review in Periodontology 2000, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers, 4 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
63 citing papers in PubMed, 4 syntheses or guidelines pooled it.
- Salivary microbial meta-analysis reveals gender differences in oral microbiota, core microbiota, and molecular markers.Frontiers in cellular and infection microbiology · 2026Pooled it
- Vitamin D as a candidate host susceptibility factor in sugar-driven oral dysbiosis.Frontiers in oral health · 2026Pooled it
- Artificial Intelligence Models for Diagnosis of Periodontitis Using Non-Invasive Biological Markers: A Systematic Review and Meta-Analysis of Patient-Based Studies.Medical sciences (Basel, Switzerland) · 2025Pooled it
- Meta-Analysis Using NGS Data: TheFrontiers in oral health · 2021Pooled it
- Population-scale characterization of the oral microbiome and associations with metabolic health.Nature communications · 2026Article
- Exploring the oral microbiome: from traditional techniques to advanced omics and databases.Antonie van Leeuwenhoek · 2026Review
- Oral microbiome signatures predict biological age and host health.Nature communications · 2026Article
- Diversity and random forest models of oral microbiomes in periodontal health using publicly available data.Journal of periodontology · 2026Article
- Comparison of microbiome profiles across sample types in patients with peri-implantitis using 16S rRNA sequencing.Journal of periodontal & implant science · 2026Article
- Distinct gut and oral microbiome patterns associated with dyslexia in a family-based cohort: A preliminary exploratory study.PloS one · 2026Article
- A two-hit ecological framework linking social context to caries-associated microbiome shifts in children.Journal of oral microbiology · 2026Article
- The Interplay Between Lifestyle and Oral/Faecal Microbial Profiles Among Periodontal Disease Patients: A Cross-Sectional Study.Journal of clinical periodontology · 2026Article
- Delayed shifts in salivary microbiota composition following long-term use of 0.06% chlorhexidine toothpaste.Frontiers in oral health · 2026Article
- Assessing the Oral Microbiome in Women of Reproductive Age: A Narrative Review.Clinics and practice · 2025Review
- Oral Microbial Diversity is Associated with Biologic Treatment and Metabolic Health Status but not with Disease Activity in Patients with Inflammatory Bowel Disease.Crohn's & colitis 360 · 2025Article
- Oral microbial profiles in young adults with cannabis use disorder.Drug and alcohol dependence · 2025Article
- Assessment of internet data on orthodontic treatment and oral hygiene in the Marmara region using Google Trends:a methodological study.European oral research · 2025Article
- SANA-Biome: A Protocol for a Cross-Sectional Study on Oral Health, Diet, and the Oral Microbiome in Romania.Healthcare (Basel, Switzerland) · 2025Article
- Article
- Plant Microbiomes Alleviate Abiotic Stress-Associated Damage in Crops and Enhance Climate-Resilient Agriculture.Plants (Basel, Switzerland) · 2025Review
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With this review, we aim to increase the quality standards for clinical studies with microbiome as an output parameter. We critically address the existing body of evidence for good quality practices in oral microbiome studies based on 16S rRNA gene amplicon sequencing. First, we discuss the usefulness of microbiome profile analyses. Is a microbiome study actually the best approach for answering the research question? This is followed by addressing the criteria for the most appropriate study design, sample size, and the necessary data (study metadata) that should be collected. Next, we evaluate the available evidence for best practices in sample collection, transport, storage, and DNA isolation. Finally, an overview of possible sequencing options (eg, 16S rRNA gene hypervariable regions, sequencing platforms), processing and data interpretation approaches, as well as requirements for meaningful data storage, sharing, and reporting are provided.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.