Evidence map›Paper›PMID 33226509›Full record

ArticleEJNMMI research2020

Plasma radio-metabolite analysis of PET tracers for dynamic PET imaging: TLC and autoradiography.

Fiona Li, Justin W Hicks, Lihai Yu, Lise Desjardin, Laura Morrison, Jennifer Hadway, Ting-Yim Lee

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in EJNMMI research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
0.4field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it, 8 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Quantitation of dynamic total-body PET imaging: recent developments and future perspectives.European journal of nuclear medicine and molecular imaging · 2023
    Review
  4. Detrimental impact of aqueous mobile phases on 18F-labelled radiopharmaceutical analysisAnalytical methods : advancing methods and applications · 2023
    Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Fiona LiDepartment of Medical Biophysics, The University of Western University, 1151 Richmond Street North, London, ON, N6A 3K7, Canada.
Justin W HicksDepartment of Medical Biophysics, The University of Western University, 1151 Richmond Street North, London, ON, N6A 3K7, Canada.
Lihai YuLawson Health Research Institute, Grosvenor Campus, 268 Grosvenor Street, London, ON, N6A 4V2, Canada.
Lise DesjardinLawson Health Research Institute, Grosvenor Campus, 268 Grosvenor Street, London, ON, N6A 4V2, Canada.
Laura MorrisonLawson Health Research Institute, Grosvenor Campus, 268 Grosvenor Street, London, ON, N6A 4V2, Canada.
Jennifer HadwayLawson Health Research Institute, Grosvenor Campus, 268 Grosvenor Street, London, ON, N6A 4V2, Canada.
Ting-Yim LeeDepartment of Medical Biophysics, The University of Western University, 1151 Richmond Street North, London, ON, N6A 3K7, Canada. tlee@imaging.robarts.ca.ORCID http://orcid.org/0000-0001-7605-9752
Lawson Health Research Institute · CAWestern University · CA

Funding

NIH HHS 1 UO1 CA190256-01
6 · The paper itself

Abstract

backgroundIn molecular imaging with dynamic PET, the binding and dissociation of a targeted tracer is characterized by kinetics modeling which requires the arterial concentration of the tracer to be measured accurately. Once in the body the radiolabeled parent tracer may be subjected to hydrolysis, demethylation/dealkylation and other biochemical processes, resulting in the production and accumulation of different metabolites in blood which can be labeled with the same PET radionuclide as the parent. Since these radio-metabolites cannot be distinguished by PET scanning from the parent tracer, their contribution to the arterial concentration curve has to be removed for the accurate estimation of kinetic parameters from kinetic analysis of dynamic PET. High-performance liquid chromatography has been used to separate and measure radio-metabolites in blood plasma; however, the method is labor intensive and remains a challenge to implement for each individual patient. The purpose of this study is to develop an alternate technique based on thin layer chromatography (TLC) and a sensitive commercial autoradiography system (Beaver, Ai4R, Nantes, France) to measure radio-metabolites in blood plasma of two targeted tracers-[

resultsRadioactivity as low as 17 Bq in 2 µL of pig's plasma can be detected on the TLC plate using autoradiography. Peaks corresponding to the parent tracer and radio-metabolites could be distinguished in the line profile through each sample (n = 8) in the autoradiographic image. Significant intersubject and intra-subject variability in radio-metabolites production could be observed with both tracers. For [

conclusionTLC coupled with autoradiography is a good alternative to high-performance liquid chromatography for radio-metabolite correction. The advantages of requiring only small blood samples (~ 100 μL) and of analyzing multiple samples simultaneously, make the method suitable for individual dynamic PET studies.

Indexed as

[18F]FAZA[18F]FEPPAAutoradiographyDynamic PETRadio-metabolite correctionThin layer chromatography (TLC)

Identifiers

PMID33226509
PMCPMC7683627
OpenAlexW3109490269

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.