ArticleEpigenetics & chromatin2020
Histone modifications associated with gene expression and genome accessibility are dynamically enriched at Plasmodium falciparum regulatory sequences.
Article in Epigenetics & chromatin, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 44 citations in OpenAlex.
- The exception that proves the rule: Virulence gene expression at the onset of Plasmodium falciparum blood stage infections.PLoS pathogens · 2023Trial
- Integration of ATAC-seq and RNA-seq reveals temperature-responsive regulatory regions in Plasmodium falciparum asexual stages.Parasites & vectors · 2026Article
- Chromatin state dynamics during the Plasmodium falciparum intraerythrocytic development cycle.BMC genomics · 2026Article
- Article
- PfGCN5 is essential for Plasmodium falciparum survival and transmission and regulates Pf H2B.Z acetylation and chromatin structure.Nucleic acids research · 2025Article
- DNA sequence and chromatin differentiate sequence-specific transcription factor binding in the human malaria parasite Plasmodium falciparum.Nucleic acids research · 2024Article
- Histone code: a common language and multiple dialects to meet the different developmental requirements of apicomplexan parasites.Current opinion in microbiology · 2024Review
- The genetic landscape of origins of replication in P. falciparum.Nucleic acids research · 2024Article
- Article
- Epigenetic Regulation and Chromatin Remodeling in Malaria Parasites.Annual review of microbiology · 2023Review
- Review
- Histone modification analysis reveals common regulators of gene expression in liver and blood stage merozoites of Plasmodium parasites.Epigenetics & chromatin · 2023Article
- Plasmodium falciparum gametocytes display global chromatin remodelling during sexual differentiation.BMC biology · 2023Article
- Patterns of Heterochromatin Transitions Linked to Changes in the Expression of Plasmodium falciparum Clonally Variant Genes.Microbiology spectrum · 2023Article
- Structural insights into acetylated histone ligand recognition by the BDP1 bromodomain of Plasmodium falciparum.International journal of biological macromolecules · 2022Article
- Review
- Relationship of circulating Plasmodium falciparum lifecycle stage to circulating parasitemia and total parasite biomass.Nature communications · 2022Article
- Distinct Histone Post-translational Modifications duringJournal of proteome research · 2022Article
- Genome-wide localization of histone variants in Toxoplasma gondii implicates variant exchange in stage-specific gene expression.BMC genomics · 2022Article
- Epigenomic signatures on paralogous genes reveal underappreciated universality of active histone codes adopted across animals.Computational and structural biotechnology journal · 2022Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
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Abstract
backgroundThe malaria parasite Plasmodium falciparum has an unusually euchromatic genome with poorly conserved positioning of nucleosomes in intergenic sequences and poorly understood mechanisms of gene regulation. Variant histones and histone modifications determine nucleosome stability and recruit trans factors, but their combinatorial contribution to gene regulation is unclear.
resultsHere, we show that the histone H3 acetylations H3K18ac and H3K27ac and the variant histone Pf H2A.Z are enriched together at regulatory sites upstream of genes. H3K18ac and H3K27ac together dynamically mark regulatory regions of genes expressed during the asexual life cycle. In contrast, H3K4me1 is depleted in intergenic sequence and dynamically depleted upstream of expressed genes. The temporal pattern of H3K27ac and H3K18ac enrichment indicates that they accumulate during S phase and mitosis and are retained at regulatory sequences until at least G1 phase and after cessation of expression of the cognate genes. We integrated our ChIPseq data with existing datasets to show that in schizont stages H3K18ac, H3K27ac and Pf H2A.Z colocalise with the transcription factor PfAP2-I and the bromodomain protein PfBDP1 and are enriched at stably positioned nucleosomes within regions of exposed DNA at active transcriptional start sites. Using transient transfections we showed that sequences enriched with colocalised H3K18ac, H3K27ac and Pf H2A.Z possess promoter activity in schizont stages, but no enhancer-like activity.
conclusionsThe dynamic H3 acetylations define P. falciparum regulatory sequences and contribute to gene activation. These findings expand the knowledge of the chromatin landscape that regulates gene expression in P. falciparum.
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