Evidence map›Paper›PMID 33225133›Full record

ArticleACS omega2020

Time-Dependent Fluorescence Spectroscopy to Quantify Complex Binding Interactions.

Samuel P Bernhard, Candace K Goodman, Erienne G Norton, Daniel G Alme, C Martin Lawrence, Mary J Cloninger

Abstract read
In one paragraph

Article in ACS omega, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
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  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Samuel P BernhardDepartment of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59718, United States.
Candace K GoodmanDepartment of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59718, United States.
Erienne G NortonDepartment of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59718, United States.
Daniel G AlmeDepartment of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59718, United States.
C Martin LawrenceDepartment of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59718, United States.
Mary J CloningerDepartment of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59718, United States.

Funding

Research Core (Developmental Research Project Program)P20GM103474 · NIGMS · MONTANA STATE UNIVERSITY - BOZEMAN · PI Jeffrey Good · 2012 to 2026
$60.0M
NIGMS NIH HHS P20 GM103474
6 · The paper itself

Abstract

Measuring the binding affinity for proteins that can aggregate or undergo complex binding motifs presents a variety of challenges. In this study, fluorescence lifetime measurements using intrinsic tryptophan fluorescence were performed to address these challenges and to quantify the binding of a series of carbohydrates and carbohydrate-functionalized dendrimers to recombinant human galectin-3. Collectively, galectins represent an important target for study; in particular, galectin-3 plays a variety of roles in cancer biology. Galectin-3 binding dissociation constants (

Identifiers

PMID33225133
PMCPMC7675582

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.